Anti-SET7 抗体 [s4E5]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SET7 antibody [s4E5] (AB14820)

Overlay histogram showing HeLa cells stained with ab14820 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14820, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

This image was produced using the ascites version of this antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SET7 antibody [s4E5] (AB14820)

Immunocytochemistry/ Immunofluorescence analysis of SET7/9 in HeLa cells. The cell was stained with ab14820 (1 : 100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SET7 antibody [s4E5] (AB14820)

Flow cytometry analysis of SET7/9 in Jurkat cells. The cell was stained with ab14820 at 2-5μg for 1x10⁶ cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-SET7 antibody [s4E5] (AB14820)

Immunoflouresence was carried out using mouse cells in culture. Conditions : 4% PFA fixation and dilution with 0.5% triton, 10mg/ml BSA in PBS. Dilution of ab14820 used : 1/50. Green = ab14820 detected with secondary antibody (Alexa 488). Blue = DAPI stain.

This image was produced using the ascites version of this antibody.

This image is courtesy of Katia Ancelin

• WB

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Western blot - Anti-SET7 antibody [s4E5] (AB14820)

All lanes:

Western blot - Anti-SET7 antibody [s4E5] (ab14820) at 1/2000 dilution

Lane 1:

293T cell lysate at 5 µg

Lane 2:

SET7/9 transfected 293T cell lysate at 5 µg

Secondary

All lanes:

goat anti-mouse secondary antibody conjugated to HRP

Predicted band size: 41 kDa

false

• WB

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Western blot - Anti-SET7 antibody [s4E5] (AB14820)

All lanes:

Western blot - Anti-SET7 antibody [s4E5] (ab14820) at 1/1000 dilution

All lanes:

Recombinant human SET7/9 protein

Secondary

All lanes:

Goat anti-mouse secondary antibody conjugated to HRP

Predicted band size: 41 kDa

false

• WB

Unknown

Western blot - Anti-SET7 antibody [s4E5] (AB14820)

Western blot analysis of recombinant SET7 using ab14820 at 1/1000. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system. Western blot analysis of recombinant SET7 using ab14820 at 1/1000. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system.

This image was produced using the ascites version of this antibody.

All lanes:

Western blot - Anti-SET7 antibody [s4E5] (ab14820)

Predicted band size: 41 kDa

false