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AB202437

Anti-SET5 抗体 [EPR20102]

Anti-SET5 antibody [EPR20102]

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(1 Publication)

Rabbit Recombinant Monoclonal SET5 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication.

別名を表示する

YHR207C, SET5, Histone-lysine N-methyltransferase SET5, SET domain-containing protein 5

2 Images
Western blot - Anti-SET5 antibody [EPR20102] (AB202437)
  • WB

Supplier Data

Western blot - Anti-SET5 antibody [EPR20102] (AB202437)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution

All lanes:

Saccharomyces cerevisiae whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

true

Exposure time: 3s

Western blot - Anti-SET5 antibody [EPR20102] (AB202437)
  • WB

Supplier Data

Western blot - Anti-SET5 antibody [EPR20102] (AB202437)

Blocking/Dilution buffer : 5% NFDM/TBST.

Set5 KO strain (Y02901) was purchased from Euroscarf.

All lanes:

Western blot - Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution

Lane 1:

Saccharomyces cerevisiae (WT) whole cell lysate at 10 µg

Lane 2:

Saccharomyces cerevisiae (SET5 KO) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

true

Exposure time: 30s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-SET5 antibody [EPR20102] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20102

アイソタイプ

IgG

キャリアフリー

No

交差種

Saccharomyces cerevisiae

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SET5 also known as lysine methyltransferase 5 (KMT5) is a histone methyltransferase enzyme with a role in epigenetic regulation. It weighs approximately 46 kDa and localizes predominantly in the nucleus. SET5 selectively methylates histone H4 at specific lysine residues which affects chromatin structure and gene expression. The expression of SET5 occurs in diverse tissues with notable activity in cells undergoing high rates of division.
Biological function summary

SET5 influences gene expression by modulating chromatin accessibility. This methyltransferase enzyme adds methyl groups to histone proteins leading to changes in chromatin compaction and transcriptional activity. SET5 does not form part of a larger protein complex but directly interacts with histone substrates to exert its effects. Its activity links to cellular processes that drive proliferation and differentiation.

Pathways

SET5 integrates into epigenetic signaling that govern transcription. It contributes to the regulation of the MAPK signaling pathway influencing cell growth and differentiation. SET5 activity also interfaces with the mitogen-activated protein kinase (MAPK) pathway where its function may interact with proteins like ERK2 in the context of gene regulation. Through these interactions SET5 plays a role in fine-tuning the cellular response to external and internal cues intended for growth and proliferation.

Alterations in SET5 function associate with cancer and developmental disorders. In cancers aberrant SET5 activity may lead to uncontrolled cellular proliferation through dysregulated transcription of key growth-related genes. Disorders such as Kabuki syndrome have connections to dysregulation in histone methylation machinery with SET5 possibly linking with MLL2 another methyltransferase implicated in the disorder. Understanding SET5's role in these conditions helps guide potential therapeutic approaches targeting chromatin modifiers.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Histone methyltransferase that monomethylates 'Lys-5', 'Lys-8' and 'Lys-12' of histone H4 (H4K5me1, H4K8me1 and H4K12me1, respectively), thereby controlling gene expression and remodeling chromatin structures (PubMed : 22343720). Functions together with the chromatin-modifying complexes COMPASS and NuA4 to regulate cell growth and stress responses (PubMed : 22343720).. (Microbial infection) Acts as a virus host factor involved in the replication of positive-strand RNA viruses like the MBV.
See full target information SET5
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Prostate 80:1145-1156 PubMed32659025

2020

CXCL12γ induces human prostate and mammary gland development.

Applications

Unspecified application

Species

Unspecified reactive species

Younghun Jung,Jin Koo Kim,Eunsohl Lee,Frank C Cackowski,Ann M Decker,Paul H Krebsbach,Russell S Taichman
View all publications
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