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AB202434

Anti-SET3 抗体 [EPR20101]

Anti-SET3 antibody [EPR20101]

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(1 Publication)

Rabbit Recombinant Monoclonal SET3 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication.

別名を表示する

YKR029C, SET3, SET domain-containing protein 3

2 Images
Western blot - Anti-SET3 antibody [EPR20101] (AB202434)
  • WB

Supplier Data

Western blot - Anti-SET3 antibody [EPR20101] (AB202434)

Blocking/Dilution buffer : 5% NFDM/TBST.

KO strain (Y07046) was purchased from Euroscarf.

All lanes:

Western blot - Anti-SET3 antibody [EPR20101] (ab202434) at 1/1000 dilution

Lane 1:

Saccharomyces cerevisiae (WT) whole cell lysate at 10 µg

Lane 2:

Saccharomyces cerevisiae (SET3 KO) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 85 kDa

true

Exposure time: 3min

Western blot - Anti-SET3 antibody [EPR20101] (AB202434)
  • WB

Supplier Data

Western blot - Anti-SET3 antibody [EPR20101] (AB202434)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SET3 antibody [EPR20101] (ab202434) at 1/2000 dilution

All lanes:

Saccharomyces cerevisiae whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 85 kDa

true

Exposure time: 3min

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-SET3 antibody [EPR20101] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20101

アイソタイプ

IgG

キャリアフリー

No

交差種

Saccharomyces cerevisiae

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SET3 also known as SET domain containing 3 is a protein with a molecular mass of approximately 98 kDa. It functions as a histone methyltransferase an enzyme that adds methyl groups to histone proteins. This action influences chromatin structure and gene expression. SET3 is expressed in various tissues including heart and skeletal muscle with notable levels in hematopoietic cells. It plays an essential role in regulating the transcriptional programs inside these cells.
Biological function summary

In the cell SET3 participates as part of a multi-protein complex known as the SET3 complex. This complex is involved in deacetylating and methylating histones which affects gene transcription levels. The modification of histones managed by the SET3 complex helps in maintaining stable gene expression patterns necessary for cell differentiation and development processes. It ensures correct chromatin organization and therefore proper transcription regulation.

Pathways

Histone modification processes involve SET3 contributing significantly to gene silencing pathways and chromatin remodeling. In gene silencing pathways SET3 works closely with histone deacetylases to remove acetyl groups therefore repressing gene expression. Chromatin remodeling involves consort with other proteins like SIN3A to control access of transcription factors to DNA. These pathways are essential for proper embryonic development and cell identity maintenance.

Disruption of SET3 expression can link to various hematological malignancies like leukemia where chromatin misregulation plays a role. Its aberrant function or expression might also connect to developmental disorders due to failure in proper gene regulation. In leukemia SET3 correlates with proteins like RUNX1 and CEBPA which are critical for hematopoietic differentiation potentially leading to hematopoietic cell transformation when improperly regulated.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcriptional regulator that acts via the formation of large multiprotein complexes that modify and/or remodel the chromatin. Required for both gene activation and repression. Part of the Set3C complex, which is required to repress early/middle sporulation genes during meiosis. Required for the transcriptional activation of genes with high activity.
See full target information SET3
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Prostate 80:1145-1156 PubMed32659025

2020

CXCL12γ induces human prostate and mammary gland development.

Applications

Unspecified application

Species

Unspecified reactive species

Younghun Jung,Jin Koo Kim,Eunsohl Lee,Frank C Cackowski,Ann M Decker,Paul H Krebsbach,Russell S Taichman
View all publications
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