AB176567

Anti-SET/TAF-I 抗体 [EPR12972(B)]

Anti-SET/TAF-I antibody [EPR12972(B)]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal SET/TAF-I antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

別名を表示する

Protein SET, HLA-DR-associated protein II, Inhibitor of granzyme A-activated DNase, PHAPII, Phosphatase 2A inhibitor I2PP2A, Template-activating factor I, IGAAD, I-2PP2A, TAF-I, SET

3 Images

Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12972(B)] (AB176567)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12972(B)] (AB176567)

Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling SET/TAF-I (red) with ab176567 at a 1/10 dilution, or negative control rabbit IgG (green)

Supplier Data

Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12972(B)] (AB176567)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling SET/TAF-I with ab176567 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)

Predicted band size: 33 kDa

true

Supplier Data

Western blot - Anti-SET/TAF-I antibody [EPR12972(B)] (AB176567)

All lanes:

Western blot - Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

true

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR12972(B)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt (Intra), IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SET/TAF-I also known as Inhibitor of Protein Phosphatase 2A (I2PP2A) is a multifunctional protein with an approximate mass of 39 kDa. It is ubiquitously expressed in various human tissues including the brain liver and testis. SET/TAF-I plays a role in chromatin remodeling and gene transcription regulation by inhibiting protein phosphatase 2A (PP2A) activity. The protein interacts with nucleosome assembly and in chromatin sliding affecting the overall structure and function of chromosomal material.

Biological function summary

In the context of cellular processes SET/TAF-I functions as a histone chaperone and is a part of the nucleosome assembly complex. This protein is involved in nucleosome disassembly and assembly during DNA replication and repair and it modulates transcriptional access for various transcription factors. It regulates the acetylation of histones influencing gene expression levels. The SET/TAF-I also participates in apoptosis and autophagy demonstrating its versatility in cellular control mechanisms.

Pathways

Studies show that SET/TAF-I engages in the MAPK/ERK signaling pathway which is critical for cell cycle progression and proliferation. It is linked with other proteins such as HMGN proteins and histone deacetylases playing a substantial role in chromatin dynamics and genomic stability. Furthermore SET/TAF-I is central to the signaling pathway that maintains normal cellular functions and responds to stress signals like oxidative stress demonstrating its importance in normal cell regulation.

Abnormalities in SET/TAF-I levels link to Alzheimer's disease and acute myeloid leukemia. In Alzheimer's the protein accumulation leads to tau hyperphosphorylation an important feature of neurodegeneration influencing PP2A's role in phosphatase activity. In acute myeloid leukemia SET/TAF-I dysregulation results in altered cell proliferation and survival signaling implicating proteins like BCL-2 and downstream apoptotic regulators. This demonstrates the protein's involvement in both neurological disorders and malignancies highlighting its broader clinical significance.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

See full target information SET

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 30:3171-3182.e6 PubMed32130916

2020

PP2A Phospho-Tyr Antibodies Are Not Specific for this Modification but Are Sensitive to Other PP2A Modifications Including Leu Methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ingrid E Frohner,Ingrid Mudrak,Stefan Schüchner,Dorothea Anrather,Markus Hartl,Jean-Marie Sontag,Estelle Sontag,Brian E Wadzinski,Teresa Preglej,Wilfried Ellmeier,Egon Ogris

Nature immunology 17:556-64 PubMed26974206

2016

Phosphatase PP2A is requisite for the function of regulatory T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sokratis A Apostolidis,Noé Rodríguez-Rodríguez,Abel Suárez-Fueyo,Nikolina Dioufa,Esra Ozcan,José C Crispín,Maria G Tsokos,George C Tsokos

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SET/TAF-I 抗体 [EPR12972(B)]

Anti-SET/TAF-I antibody [EPR12972(B)]

Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12972(B)] (AB176567)

Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12972(B)] (AB176567)

Western blot - Anti-SET/TAF-I antibody [EPR12972(B)] (AB176567)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise