Anti-Seryl-tRNA synthetase/SERS 抗体 [EPR10684(B)] (ab154825)
![Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)](https://www.abcam.co.jp/ps/products/154/ab154825/Images/ab154825-161397-anti-seryl-trna-synthetase-antibody-epr10684b-western-blot.jpg "Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10684(B)] to Seryl-tRNA synthetase/SERS
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)]
Seryl-tRNA synthetase/SERS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10684(B)] to Seryl-tRNA synthetase/SERS -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human Seryl-tRNA synthetase/SERS (N terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- 293T and K562 cell lysates; Human pancreas tissue and Human thyroid gland carcinoma tissue; 293T cells and K562 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10684(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154825の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Heat to 98°C, allow to cool for 10-20 minutes) |
|
| ICC/IF |
1/100 - 1/250.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
|
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). -
パスウェイ
Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. -
配列類似性
Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily. -
ドメイン
Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 6301 Human
- Entrez Gene: 20226 Mouse
- Entrez Gene: 266975 Rat
- Omim: 607529 Human
- SwissProt: P49591 Human
- SwissProt: P26638 Mouse
- SwissProt: Q6P799 Rat
- Unigene: 531176 Human
see all -
別名
- C78314 antibody
- cytoplasmic antibody
- EC 6.1.1.11 antibody
see all
画像
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Western blot - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)All lanes : Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 59 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)](https://www.abcam.co.jp/ps/products/154/ab154825/Images/ab154825-161396-anti-seryl-trna-synthetase-antibody-epr10684b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)](https://www.abcam.co.jp/ps/products/154/ab154825/Images/ab154825-161395-anti-seryl-trna-synthetase-antibody-epr10684b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)](https://www.abcam.co.jp/ps/products/154/ab154825/Images/ab154825-161394-anti-seryl-trna-synthetase-antibody-epr10684b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)Immunofluorescence analysis of 293T cells labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/100 dilution.
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![Flow Cytometry (Intracellular) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)](https://www.abcam.co.jp/ps/products/154/ab154825/Images/ab154825-161393-anti-seryl-trna-synthetase-antibody-epr10684b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)Intracellular flow cytometric analysis of permeabilized K562 cells labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/100 dilution (red) or a negative Rabbit IgG (green).
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![Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)](https://www.abcam.co.jp/ps/products/154/ab154825/Images/ab154825-6-benefits-of-recombinant-antibodies.png)
Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab154825 は 4 報の論文で使用されています。
- He J et al. Heterozygous Seryl-tRNA Synthetase 1 Variants Cause Charcot-Marie-Tooth Disease. Ann Neurol 93:244-256 (2023). PubMed: 36088542
- Dai W et al. ASCT2 Is Involved in SARS-Mediated ß-Casein Synthesis of Bovine Mammary Epithelial Cells with Methionine Supply. J Agric Food Chem 68:13038-13045 (2020). PubMed: 31597423
- Dai WT et al. Seryl-tRNA synthetase-mediated essential amino acids regulate ß-casein synthesis via cell proliferation and mammalian target of rapamycin (mTOR) signaling pathway in bovine mammary epithelial cells. J Dairy Sci 101:10456-10468 (2018). PubMed: 30219419
- Xu L et al. Three distinct 3-methylcytidine (m3C) methyltransferases modify tRNA and mRNA in mice and humans. J Biol Chem 292:14695-14703 (2017). PubMed: 28655767