Anti-Serum Amyloid P/SAP 抗体 [EP1018Y] (ab45151)
![Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)](https://www.abcam.co.jp/ps/products/45/ab45151/Images/ab45151-266211-anti-serum-amyloid-p-sap-antibody-ep1018y-western-blot.jpg "Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1018Y] to Serum Amyloid P/SAP
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Serum Amyloid P/SAP antibody [EP1018Y]
Serum Amyloid P/SAP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1018Y] to Serum Amyloid P/SAP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Serum Amyloid P/SAP aa 1 to the C-terminus (N terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- WB: Human serum; Human liver lysate. IHC-P: Human endometrial carcinoma tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1018Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45151の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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| IHC-P |
1/50 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Enzymatic antigen retrieval (using protease) also has been found to work and may acheive better results |
| 特記事項 |
|---|
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WB
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
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IHC-P
1/50 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Enzymatic antigen retrieval (using protease) also has been found to work and may acheive better results |
ターゲット情報
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機能
Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. -
組織特異性
Found in serum and urine. -
関連疾患
Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. -
配列類似性
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
翻訳後修飾
N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148). -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 325 Human
- Omim: 104770 Human
- SwissProt: P02743 Human
- Unigene: 507080 Human
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別名
- 9.5S alpha 1 glycoprotein antibody
- 9.5S alpha-1-glycoprotein antibody
- Amyloid P component serum antibody
see all
画像
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Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) at 1/10000 dilution + Human serum at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 25 kDaDiluting and blocking buffer: 5% NFDM /TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)](https://www.abcam.co.jp/ps/products/45/ab45151/Images/ab45151-266212-anti-serum-amyloid-p-sap-antibody-ep1018y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)ab45151 staining Serum Amyloid P/SAP in human endometrial carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
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![Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)](https://www.abcam.co.jp/ps/products/45/ab45151/Images/ab45151-266210-anti-serum-amyloid-p-sap-antibody-ep1018y-western-blot.jpg)
Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) at 1/5000 dilution + Human liver at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 25 kDaBlocking and diluting buffer: 5% NFDM /TBST
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![Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)](https://www.abcam.co.jp/ps/products/45/ab45151/Images/ab45151-6-benefits-of-recombinant-antibodies.png)
Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab45151 は 11 報の論文で使用されています。
- Lee JH et al. Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis. Clin Proteomics 17:20 (2020). PubMed: 32518534
- Bergkvist L et al. Serum amyloid P component promotes formation of distinct aggregated lysozyme morphologies and reduces toxicity in Drosophila flies expressing F57I lysozyme. PLoS One 15:e0227227 (2020). PubMed: 31978114
- Bai B et al. Deep Multilayer Brain Proteomics Identifies Molecular Networks in Alzheimer's Disease Progression. Neuron 105:975-991.e7 (2020). PubMed: 31926610
- Girton AW et al. Serum Amyloid P and IgG Exhibit Differential Capabilities in the Activation of the Innate Immune System in Response to Bacillus anthracis Peptidoglycan. Infect Immun 86:N/A (2018). PubMed: 29531132
- Helmfors L et al. Serum Amyloid P Component Ameliorates Neurological Damage Caused by Expressing a Lysozyme Variant in the Central Nervous System of Drosophila melanogaster. PLoS One 11:e0159294 (2016). IHC-P . PubMed: 27428539