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AB232468

Anti-Serum Amyloid P/SAP 抗体 [EP1018Y] - BSA and Azide free

Anti-Serum Amyloid P/SAP antibody [EP1018Y] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Serum Amyloid P/SAP antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

PTX2, APCS, Serum amyloid P-component, SAP, 9.5S alpha-1-glycoprotein

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] - BSA and Azide free (AB232468)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] - BSA and Azide free (AB232468)

ab45151 staining Serum Amyloid P/SAP in human endometrial carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1 : PBS in place of primary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45151).

関連する標識済み抗体及び組成の異なる製品 (3)

  • Unconjugated

    Anti-Serum Amyloid P/SAP antibody [EP1018Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Serum Amyloid P/SAP antibody [EP1018Y]

  • HRP

    HRP Anti-Serum Amyloid P/SAP antibody [EP1018Y]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1018Y

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB268071

Anti-Serum Amyloid P/SAP antibody [APCS/3240]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [APCS/3240] (AB268071)

  • 0
  • 0
Primary Antibodies

AB27313

Anti-Serum Amyloid P/SAP antibody [14B4]

Western blot - Anti-Serum Amyloid P/SAP antibody [14B4] (AB27313)

  • 5
  • 2
Primary Antibodies

AB233265

Anti-Serum Amyloid P/SAP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody (AB233265)

  • 0
  • 0

組合せ製品

Assay Kits

AB246537

Human SAP ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human SAP ELISA Kit (AB246537)

  • 0
  • 0
Primary Antibodies

AB201811

Anti-PD1 antibody [NAT105] - BSA and Azide free

BOND RX™ Validated|Recombinant

Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (AB201811)

  • 0
  • 0
Primary Antibodies

AB32520

Anti-STAT6 antibody [YE361]

20ul selling size|Recombinant|Advanced Validation|RabMAb|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (AB32520)

  • 5
  • 1
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB53490

Anti-PAX8 antibody [PAX8R1]

Immunocytochemistry - Anti-PAX8 antibody [PAX8R1] (AB53490)

  • 3
  • 6
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Enzymatic antigen retrieval (using protease) also has been found to work and may acheive better results.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

ab232468 is the carrier-free version of ab45151.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid P component also known as SAP P-SAP or APCS protein is a pentraxin family member. It has a molecular mass of approximately 25 kDa. SAP expresses mainly in the liver and circulates in blood plasma. The protein consists of five identical subunits which form a homopentameric structure. This structure enables SAP to bind to amyloid fibrils a defining mechanical action of this protein.
Biological function summary

SAP plays a role in the immune system by recognizing damaged cells and pathogens. It acts as an opsonin marking these cellular targets for phagocytosis. As part of a complex SAP stabilizes extracellular matrix and amyloid deposits. It binds specifically to amyloid fibrils found in various tissues potentially facilitating their removal by immune cells. The protein's binding actions are important for its role in maintaining tissue and fluid homeostasis.

Pathways

SAP is actively involved in the innate immune response and amyloidogenic pathways. It interacts with the complement system to enhance the clearance of cell debris and amyloid fibrils. The complement system is an important component of innate immunity where SAP can engage with complement proteins like C1q. This interaction suggests that SAP plays roles in modulating both inflammation and tissue repair processes.

SAP has significant connections. SAP is related to systemic amyloidosis where it binds to amyloid deposits affecting multiple organs. Moreover SAP has been implicated in Alzheimer's disease due to its interaction with beta-amyloid protein plaques. In these disorders the involvement of SAP aids researchers in understanding the pathological mechanisms and could open avenues for targeted therapies aimed at modulating SAP functions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
See full target information APCS
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 10: PubMed36009539

2022

Autologous Platelet-Rich Growth Factor Reduces M1 Macrophages and Modulates Inflammatory Microenvironments to Promote Sciatic Nerve Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Anjali Yadav,Thamil Selvee Ramasamy,Sheng-Che Lin,Szu-Han Chen,Jean Lu,Ya-Hsin Liu,Fu-I Lu,Yuan-Yu Hsueh,Shau-Ping Lin,Chia-Ching Wu
View all publications
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Abcam product promise

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