AB190802

Anti-Serum Amyloid A 抗体 [EP11592-92]

Anti-Serum Amyloid A antibody [EP11592-92]

20ul selling size
BOND RX™ Validated
RabMAb
Recombinant
詳細を見る

(1 Review)

(5 Publications)

Anti-Serum Amyloid A antibody [EP11592-92] (ab190802) is a rabbit monoclonal antibody detecting Serum Amyloid A in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

Serum amyloid A-1 protein, SAA, SAA1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid A antibody [EP11592-92] (AB190802)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid A antibody [EP11592-92] (AB190802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue sections labeling Serum Amyloid A with Purified ab190802 at 1 : 400 dilution (0.96 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Unknown

Western blot - Anti-Serum Amyloid A antibody [EP11592-92] (AB190802)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Serum Amyloid A antibody [EP11592-92] (ab190802) at 1/1000 dilution

All lanes:

Human plasma lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 14 kDa

Observed band size: 12 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP11592-92

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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製品の詳細

What is this antibody validated in?
Anti-Serum Amyloid A antibody [EP11592-92] (ab190802) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Serum Amyloid A?
Anti-Serum Amyloid A [EP11592-92] (ab190802) specifically detects a band for Serum Amyloid A (UniProt: P0DJI8) at a molecular weight of 14kDa.

Other related products
We have a range of other formats of antibody clone [EP11592-92] also available for your convenience: ab190802, Carrier free - ab235995

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid A (SAA) is an important acute-phase protein with a mass of approximately 12 kDa. It plays a role in inflammation and is present in the serum. SAA is an apolipoprotein that associates with high-density lipoprotein (HDL) during inflammatory states. The protein is expressed mainly in the liver and circulating SAA levels greatly increase during inflammatory responses marking it as an important marker for inflammation.

Biological function summary

SAA contributes to the body's immune response to injury by recruiting immune cells and facilitating the removal of cholesterol. As part of the acute-phase response SAA integrates into HDL displacing other apolipoproteins and altering HDL's function. Increased SAA levels can also modulate prostaglandin synthesis and secretion influencing the inflammatory response. The exact role of SAA in pathways beyond these functions continues to be a subject of research.

Pathways

SAA influences lipid metabolism and inflammatory pathways. It plays a significant role within the acute-phase response pathway which modulates the immune system's reaction to stress such as infection or injury. Another pathway involves cholesterol metabolism where SAA interacts with apolipoproteins AI and AII altering the composition and function of HDL particles.

High levels of SAA associate with amyloidosis and chronic inflammatory diseases. In amyloidosis misfolded SAA proteins deposit as amyloid fibrils in tissues leading to organ dysfunction. In dogs and other species SAA is used as a biomarker to monitor inflammatory diseases. Its connection with C-reactive protein further emphasizes its role in inflammatory processes. Research continues to explore how modulating SAA levels may impact disease progression and treatment.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Major acute phase protein.

See full target information SAA1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 32:1921-1934 PubMed39574472

2024

Using Multi-Omics Analysis to Explore Diagnostic Tool and Optimize Drug Therapy Selection for Patients with Glioma Based on Cross-Talk Gene Signature.

Applications

Unspecified application

Species

Unspecified reactive species

Yushi Yang,Chujiao Hu,Shan Lei,Xin Bao,Zhirui Zeng,Wenpeng Cao

Cell research 33:585-603 PubMed37337030

2023

An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wu,Jiayan Yan,Yinqi Bai,Feiyu Chen,Xuanxuan Zou,Jiangshan Xu,Ao Huang,Liangzhen Hou,Yu Zhong,Zehua Jing,Qichao Yu,Xiaorui Zhou,Zhifeng Jiang,Chunqing Wang,Mengnan Cheng,Yuan Ji,Yingyong Hou,Rongkui Luo,Qinqin Li,Liang Wu,Jianwen Cheng,Pengxiang Wang,Dezhen Guo,Waidong Huang,Junjie Lei,Shang Liu,Yizhen Yan,Yiling Chen,Sha Liao,Yuxiang Li,Haixiang Sun,Na Yao,Xiangyu Zhang,Shiyu Zhang,Xi Chen,Yang Yu,Yao Li,Fengming Liu,Zheng Wang,Shaolai Zhou,Huanming Yang,Shuang Yang,Xun Xu,Longqi Liu,Qiang Gao,Zhaoyou Tang,Xiangdong Wang,Jian Wang,Jia Fan,Shiping Liu,Xinrong Yang,Ao Chen,Jian Zhou

International journal of dermatology 61:e398-e400 PubMed35304754

2022

High levels of serum amyloid A in patients with generalized pustular psoriasis: a possible biomarker of generalized pustular psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuhiko Mori,Toshiyuki Yamamoto

Scientific reports 11:2291 PubMed33504824

2021

Altered high-density lipoprotein composition and functions during severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Floran Begue,Sébastien Tanaka,Zarouki Mouktadi,Philippe Rondeau,Bryan Veeren,Nicolas Diotel,Alexy Tran-Dinh,Tiphaine Robert,Erick Vélia,Patrick Mavingui,Marie Lagrange-Xélot,Philippe Montravers,David Couret,Olivier Meilhac

International journal of molecular medicine 41:61-68 PubMed29115584

2017

Apolipoprotein C-III in the high-density lipoprotein proteome of cerebral lacunar infarction patients impairs its anti-inflammatory function.

Applications

Unspecified application

Species

Unspecified reactive species

Pu Lv,Mingming Zhao,Yuanyuan Liu,Haiqiang Jin,Wei Cui,Chenghe Fan,Yuming Teng,Lemin Zheng,Yining Huang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Serum Amyloid A 抗体 [EP11592-92]

Anti-Serum Amyloid A antibody [EP11592-92]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid A antibody [EP11592-92] (AB190802)

Western blot - Anti-Serum Amyloid A antibody [EP11592-92] (AB190802)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

Abcam product promise