Anti-SERPINE2/PN-1 抗体 (ab154591)
Key features and details
- Rabbit polyclonal to SERPINE2/PN-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SERPINE2/PN-1 antibody
SERPINE2/PN-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SERPINE2/PN-1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human SERPINE2/PN-1 aa 163-385.
Database link: P07093 -
ポジティブ･コントロール
- HeLa, HepG2 and Mouse brain whole cell lysates; HeLa cells; Human gastric carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154591の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
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| IHC-P |
1/100 - 1/1000.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa. |
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IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin. -
配列類似性
Belongs to the serpin family. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 5270 Human
- Entrez Gene: 20720 Mouse
- Omim: 177010 Human
- SwissProt: P07093 Human
- SwissProt: Q07235 Mouse
- Unigene: 38449 Human
- Unigene: 708453 Human
- Unigene: 3093 Mouse
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別名
- GDN antibody
- GDN_HUMAN antibody
- GDNPF antibody
see all
画像
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Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)All lanes : Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
10% SDS PAGE -
Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SERPINE2/PN-1 antibody (ab154591)Immunofluorescence analysis of methanol-fixed HeLa cells labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINE2/PN-1 antibody (ab154591)Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab154591 は 4 報の論文で使用されています。
- Lin K et al. Combining single-cell transcriptomics and CellTagging to identify differentiation trajectories of human adipose-derived mesenchymal stem cells. Stem Cell Res Ther 14:14 (2023). PubMed: 36721241
- Pan XW et al. Molecular subtyping and characterization of clear cell renal cell carcinoma by tumor differentiation trajectories. iScience 26:108370 (2023). PubMed: 38034348
- Li YB et al. miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2. Mol Med Rep 16:2417-2424 (2017). PubMed: 28677735
- Wu X et al. The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration. Sci Rep 6:30563 (2016). PubMed: 27460424