Anti-SERPINB1/PI2 抗体 (ab47731)
Key features and details
- Rabbit polyclonal to SERPINB1/PI2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SERPINB1/PI2 antibody
SERPINB1/PI2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SERPINB1/PI2 -
由来種
Rabbit -
特異性
This antibody is specific for SERPINB1/PI2.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human SERPINB1/PI2 (N terminal).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47731の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. -
配列類似性
Belongs to the serpin family. Ov-serpin subfamily. -
ドメイン
Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1992 Human
- Omim: 130135 Human
- SwissProt: P30740 Human
- Unigene: 381167 Human
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別名
- Anti elastase antibody
- EI antibody
- ELANH2 antibody
see all
画像
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Western blot - Anti-SERPINB1/PI2 antibody (ab47731)All lanes : Anti-SERPINB1/PI2 antibody (ab47731) at 1/1000 dilution
All lanes : MH-S cell lysate
Lysates/proteins at 20 µl per lane.
Predicted band size: 43 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa (possible degradation product) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody (ab47731)ab47731 (2µg/ml) staining Serpin B1 in human spleen using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining in both red and white pulp.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab47731 は 5 報の論文で使用されています。
- Sommeregger W et al. Proteomic differences in recombinant CHO cells producing two similar antibody fragments. Biotechnol Bioeng 113:1902-12 (2016). WB . PubMed: 26913574
- van Kempen PM et al. Oropharyngeal squamous cell carcinomas differentially express granzyme inhibitors. Cancer Immunol Immunother 65:575-85 (2016). PubMed: 26993499
- Vermeulen JF et al. Pediatric Primitive Neuroectodermal Tumors of the Central Nervous System Differentially Express Granzyme Inhibitors. PLoS One 11:e0151465 (2016). PubMed: 26963506
- Uchiyama K et al. Serpin B1 protects colonic epithelial cell via blockage of neutrophil elastase activity and its expression is enhanced in patients with ulcerative colitis. Am J Physiol Gastrointest Liver Physiol 302:G1163-70 (2012). WB, IHC-P ; Human . PubMed: 22421620
- Tseng MY et al. Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma. Oral Oncol 45:771-6 (2009). WB, IHC-P ; Human . PubMed: 19213596