Anti-Serine/threonine-protein kinase 4/MST-1 抗体 [EP1465Y] (ab51134)
![Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)](https://www.abcam.co.jp/ps/products/51/ab51134/Images/ab51134-389719-anti-serinethreonine-protein-kinase-4mst-1-antibody-ep1465y-western-blot-human-lysate.jpg "Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1465Y] to Serine/threonine-protein kinase 4/MST-1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y]
Serine/threonine-protein kinase 4/MST-1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1465Y] to Serine/threonine-protein kinase 4/MST-1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Serine/threonine-protein kinase 4/MST-1 aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- WB: Jurkat, Ramos and HeLa cell lysate and mouse spleen, rat spleen and human urinary bladder tissues. ICC/IF: Raw264.7 cells. IHC-P:Human gastric carcinoma. IP: Jurkat cell lysate. Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1465Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free (ab232551)
- PE Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab317916)
- APC Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab317957)
- Alexa Fluor® 488 Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab317998)
- Alexa Fluor® 594 Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab318040)
- Alexa Fluor® 555 Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab318082)
- Alexa Fluor® 647 Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab318123)
- Alexa Fluor® 750 Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab321643)
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51134の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
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| WB |
1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 56 kDa).
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| IHC-P |
1/50 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
We strongly recommend that customers perform an antigen retrieval step. |
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| ICC/IF |
1/100 - 1/250.
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| IP |
1/30 - 1/100.
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| 特記事項 |
|---|
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Flow Cyt (Intra)
1/50. |
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WB
1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 56 kDa). |
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IHC-P
1/50 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. We strongly recommend that customers perform an antigen retrieval step. |
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ICC/IF
1/100 - 1/250. |
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IP
1/30 - 1/100. |
ターゲット情報
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機能
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Contains 1 SARAH domain. -
翻訳後修飾
Autophosphorylated on serine and threonine residues. -
細胞内局在
Cytoplasm. Nucleus. The caspase-cleaved form cycles between the nucleus and cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 6789 Human
- Entrez Gene: 58231 Mouse
- Entrez Gene: 311622 Rat
- Omim: 604965 Human
- SwissProt: Q13043 Human
- SwissProt: Q9JI11 Mouse
- Unigene: 472838 Human
- Unigene: 479158 Mouse
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別名
- Kinase responsive to stress antibody
- Krs2 antibody
- Mammalian STE20 like protein kinase 1 antibody
see all
画像
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Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)All lanes : Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : STK4 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Ramos cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDaLanes 1-4: Merged signal (red and green). Green - ab51134 observed at 52 kDa. Red - loading control ab8245 observed at 37 kDa.
ab51134 Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] was shown to specifically react with MST-1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265442 (knockout cell lysate ab258215) was used. Wild-type and MST-1 knockout samples were subjected to SDS-PAGE. ab51134 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)](https://www.abcam.co.jp/ps/products/51/ab51134/Images/ab51134-266236-anti-serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-western-blot.jpg)
Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)All lanes : Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134) at 1/50000 dilution
Lane 1 : Jurkat (human acute T cell leukemia) whole cell lysate
Lane 2 : Mouse spleen
Lane 3 : Rat spleen
Lysates/proteins at 1/20 dilution per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 56 kDaDiluting and blocking buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)](https://www.abcam.co.jp/ps/products/51/ab51134/Images/ab51134-266245-anti-serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)ab51134 staining Serine/threonine-protein kinase 4/MST-1 in Raw264.7 (mouse abelson murine leukemia virus-induced tumor) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a concentration of 1/1000. ab7291 anti-Tubulin (mouse mAb) (1/1000) and ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000) were used as counterstains for primary antibody ab51134 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)](https://www.abcam.co.jp/ps/products/51/ab51134/Images/ab51134-266239-anti-serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)ab51134 staining Serine/threonine-protein kinase 4/MST-1 in human gastric carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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![Flow Cytometry (Intracellular) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)](https://www.abcam.co.jp/ps/products/51/ab51134/Images/ab51134-271131-anti-serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)Intracellular Flow Cytometry analysis of HeLa cells labelling Serine/theronine-protein kinase 4/MST-1 with purified ab51134 at a dilution of 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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![Immunoprecipitation - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)](https://www.abcam.co.jp/ps/products/51/ab51134/Images/ab51134-266247-anti-serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)ab51134 immunoprecipitating Serine/threonine-protein kinase 4/MST-1. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.
Lane 1: Jurkat (human acute T cell leukemia) whole cell lysate (10ug)
Lane 2: Jurkat (human acute T cell leukemia) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab51134 in Jurkat (human acute T cell leukemia) whole cell lysate -
![Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)](https://www.abcam.co.jp/ps/products/51/ab51134/Images/ab51134-266234-anti-serine-threonine-protein-kinase-4-mst-1-antibody-ep1465y-western-blot.jpg)
Western blot - Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134)Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] (ab51134) at 1/10000 dilution + HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 56 kDaDiluting and blocking buffer: 5% NFDM/TBST
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab51134 は 29 報の論文で使用されています。
- Fang W et al. Upregulated GPRC5A disrupting the Hippo pathway promotes the proliferation and migration of pancreatic cancer cells via the cAMP-CREB axis. Discov Oncol 14:17 (2023). PubMed: 36735162
- Zhang T et al. Mammalian Ste20-like kinase 1 inhibition as a cellular mediator of anoikis in mouse bone marrow mesenchymal stem cells. World J Stem Cells 15:90-104 (2023). PubMed: 37007455
- Huang Z et al. Mammalian Sterile 20-Like Kinase 1 Mediates Neuropathic Pain Associated with Its Effects on Regulating Mitophagy in Schwann Cells. Oxid Med Cell Longev 2022:3458283 (2022). PubMed: 35656021
- Huang Y et al. Deficiency of MST1 in endometriosis related peritoneal macrophages promoted the autophagy of ectopic endometrial stromal cells by IL-10. Front Immunol 13:993788 (2022). PubMed: 36263059
- Bata N et al. Inhibitors of the Hippo Pathway Kinases STK3/MST2 and STK4/MST1 Have Utility for the Treatment of Acute Myeloid Leukemia. J Med Chem 65:1352-1369 (2022). PubMed: 34807584