AB200833

Anti-Serine racemase 抗体 [EPR16682]

Anti-Serine racemase antibody [EPR16682]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Serine racemase antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

別名を表示する

Serine racemase, D-serine ammonia-lyase, D-serine dehydratase, L-serine ammonia-lyase, L-serine dehydratase, SRR

3 Images

Supplier Data

Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (AB200833)

Serine racemase was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HEK293 whole cell lysate 10 μg (Input). Lane 2 : ab200833 IP in HEK293 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200833 in HEK293 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds

All lanes:

Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833)

Predicted band size: 37 kDa

false

Exposure time: 30s

Supplier Data

Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (AB200833)

Serine racemase was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : LNCaP whole cell lysate 10 μg (Input). Lane 2 : ab200833 IP in LNCaP whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200833 in LNCaP whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833)

Predicted band size: 37 kDa

false

Supplier Data

Western blot - Anti-Serine racemase antibody [EPR16682] (AB200833)

Blocking/Dilution Buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Serine racemase antibody [EPR16682] (ab200833) at 1/5000 dilution

Lane 1:

LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg

Lane 2:

Human fetal brain tissue lysate at 20 µg

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

Lane 4:

HEK-293 (Human embryonic kidney) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16682

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Serine racemase also known as SRR is an enzyme involved in the conversion of L-serine to D-serine a process that helps regulate neural signaling. The enzyme has a molecular mass of approximately 37 kDa and is expressed highly in the brain specifically in regions such as the hippocampus and cortex. It utilizes pyridoxal phosphate (PLP) as a cofactor to catalyze the racemization and in certain conditions it also exhibits dehydratase activity converting serine to pyruvate and ammonia.

Biological function summary

Serine racemase plays an important role in the central nervous system. D-serine the product of its action serves as a co-agonist with glutamate at the NMDA receptor which is important for synaptic plasticity and memory formation. Serine racemase also associates with proteins like PICK1 and Golga3 affecting its localization and activity within cells. The regulation of D-serine levels impacts neurotransmission and neuron viability.

Pathways

Serine racemase is integral to the serine metabolism and NMDA receptor signaling pathways. It interacts with the enzyme glycine decarboxylase which is involved in glycine metabolism demonstrating its role in amino acid regulation. Its activity connects to the glutamatergic neurotransmission pathway influencing calcium influx and downstream signaling cascades that are essential for long-term potentiation and neuroplasticity.

Alterations in serine racemase activity or expression have been linked to conditions such as schizophrenia and Alzheimer's disease. In schizophrenia disruptions in D-serine levels possibly through interactions with proteins like NMDA receptor and D-amino acid oxidase can lead to impaired NMDA receptor function. In Alzheimer's disease abnormal D-serine metabolism is associated with neurodegeneration often involving proteins like amyloid-beta which exacerbates disease pathology.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the synthesis of D-serine from L-serine (PubMed : 20106978, PubMed : 23391306, PubMed : 29277459). D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine (By similarity).

See full target information Serine racemase

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 17: PubMed38543077

2024

Targeting MutT Homolog 1 (MTH1) for Breast Cancer Suppression by Using a Novel MTH1 Inhibitor MA-24 with Tumor-Selective Toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Kang,Jun Ma,Yuling Hu,Rongrong Di,Lei Wang,Xuanling Zhang,Yisheng Lai,Yu Liu

Nature metabolism 2:81-96 PubMed32694681

2020

Serine racemase enhances growth of colorectal cancer by producing pyruvate from serine.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Ohshima,Satoshi Nojima,Shinichiro Tahara,Masako Kurashige,Keisuke Kawasaki,Yumiko Hori,Moyu Taniguchi,Yutaka Umakoshi,Daisuke Okuzaki,Naoki Wada,Jun-Ichiro Ikeda,Eiichiro Fukusaki,Eiichi Morii

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Serine racemase 抗体 [EPR16682]

Anti-Serine racemase antibody [EPR16682]

Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (AB200833)

Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (AB200833)

Western blot - Anti-Serine racemase antibody [EPR16682] (AB200833)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise