Anti-SERCA2 ATPase 抗体 [IID8] (ab2817)

Selon nos informations vous avez connu quelques difficultés avec ab2817 et avez contacté notre service scientifique.

Après étude de notre correspondance, nous remarquons que nous sommes toujours en attente du questionnaire qui nous permettrait de vous aider à résoudre ce problème. Si vous avez déjà envoyé ce questionnaire, il semble que celui-ci n’est pas était pris en compte par notre système et je vous serais gré de me le transmettre de nouveau. Veuillez nous excuser par le désagrément causé par cette mesure.

Dans le cas contraire, pourriez-vous me faire savoir si vous avez déjà résolu ce problème et ainsi nous pourrions clore ce dossier dans notre système.

Je vous souhaite bonne chance dans vos recherches. N’hésitez pas à me recontacter pour plus d’assistance.

Je suis désolée d'apprendre que ab2817 ne vous donne pas de bons résultats en Western blot sur du tissu humain ou murin.

Veuillez trouver ci-joint un questionnaire discuté au téléphone qui nous permet de regrouper le plus d'informations possible sur le protocole que vous avez utilisé avec cet anticorps.
Celui-ci nous permettra de vous fournir la meilleure assistance technique.

Si nous concluons que notre produit est fautif et ne fonctionne pas comme explicité par notre fiche technique, nous serons ravis de mettre en place l'envoi d'un tube de remplacement gratuit ou d'un remboursement (sous réserve d'une commande ultérieure de 180 jours).

Je vous remercie par avance de nous envoyer votre protocole.

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. I look forward to receiving your reply.  

Thank you for your reply. From the information you have provided, the alignment with the Danio sounds high, and although we cannot guarantee it without laboratory testing, we would suggest the antibody should detect in this fish. I can recommend to consider submitting an Abreview with the results via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. We provide Abpoint rewards for submission of all Abreviews. To find out more about our Abreview system, please see the following webpage: https://www.abcam.com/abreviews I wish you the best of luck with your experiments and research. I hope this has been helpful to you. If you have any questions, please do not hesitate to contact us.

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Thank you for taking the time to complete our technical questionnaire. Our mouse monoclonal [IID8] to SERCA2 ATPase (ab2817) has been applied in many publications and we have received relatively few complaints about it. At this stage I would like to make the following recommendations: We recommend that the antibody is applied by western blot at a dilution of 1/2500. Therefore in order to improve the specificity of the antibody I would like to recommend that you reduce the dilution that you have been applying this antibody. This often serves to reduce the intensity of extraneous bands. In order to further reduce non specific bands I would like to recommend that you also reduce the mass of protein that you have been loading from 100ug to ~30ug of extract. This should be more than sufficient to detect this protein. I would also like to suggest that you change the blocking agent from milk to 3% BSA and supplement your antibody incubations with this agent. We often find that this serves to reduce background and/or extraneous bands that may be present. Please get back in touch with me should this not serve to improve your results.

Thank you for your enquiry. Regarding ab2817 and ab2861: These monoclonal antibodies have not been epitope mapped, therefore the epitope could be anywhere along the full length of the protein. I did an alignment of Human SERCA2 with the Xenopus SERCA2 sequence you attached to your email, and they are 92% homologous. I am not sure if this information helps you in your decision, but I thought I would let you know. I am still looking into ab3625 to see if I can uncover more information about the peptide that was used to produce this antibody. Once I find anything more, I will let you know. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

I'm sorry to hear you are having problems with ab2817 and ab6495. Your detailed protocol was very useful to understand the source of the problem and it is clear your protocol and modifications were very thorough. My suspicions therefore lied with your samples, they are sheep derived and neither antibody has been tested in sheep. I therefore I checked the homology between the immunogen of ab2827 (canine protein) and the sheep protein and I found only 40% homology (119 aa out of 295aa), so I suspect that the antibody ab2827 is not detecting the protein. For ab6495, the homology between the rabbit NCX1 protein and the sheep NCX1 protein was so low that I couldn't find it when BLASTing with the rabbit protein the sheep genome. So I'm sorry to say that it seems that neither antibodies seem to work due to the nature of the samples (on the protocol aspect, ab6495 was tested at a 1:200 dilution with using 5S-protein A; with ECL dilutions may need to be considerably higher regardless of the samples). Please let me know if I can be of further assistance,