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AB2818

Anti-SERCA1 ATPase 抗体 [IIH11]

Anti-SERCA1 ATPase antibody [IIH11]

4

(3 Reviews)

|

(21 Publications)

Mouse Recombinant Monoclonal SERCA1 ATPase antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 21 publications.

別名を表示する

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, SERCA1, SR Ca(2+)-ATPase 1, Calcium pump 1, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, Atp2a1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (AB2818)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (AB2818)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.177μg/ml, followed by ready to use secondary. Cytoplasmic staining on rat skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (AB2818)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (AB2818)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.117μg/ml, followed by ready to use secondary. Cytoplasmic staining on mouse skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

Western blot - Anti-SERCA1 ATPase antibody [IIH11] (AB2818)
  • WB

Supplier Data

Western blot - Anti-SERCA1 ATPase antibody [IIH11] (AB2818)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SERCA1 ATPase antibody [IIH11] (ab2818) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

false

Exposure time: 48s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

IIH11

アイソタイプ

IgG1

キャリアフリー

No

交差種

Mouse, Rat

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

エピトープ

This antibody recognizes an epitope between amino acids 199-505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.466 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.177 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.466 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.177 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SERCA1 ATPase also known as sarco/endoplasmic reticulum Ca2+ ATPase 1 is an important enzyme responsible for the active transport of Ca2+ ions from the cytosol into the sarcoplasmic reticulum which is important for muscle relaxation. This protein has a molecular weight of about 110 kDa. SERCA1 ATPase predominantly expresses in fast-twitch skeletal muscle allowing these muscles to relax rapidly after contraction. The enzyme utilizes ATP to pump calcium ions which highlights its role as an ATPase protein and its functionality in maintaining calcium homeostasis.
Biological function summary

SERCA1 ATPase ensures proper calcium regulation and muscle function by facilitating the reuptake of Ca2+ ions into the sarcoplasmic reticulum following muscle contraction. It does not operate as part of a complex but plays a significant role in calcium ion translocation thereby regulating muscle contraction-relaxation cycles. This ATPase protein is directly involved in muscle physiology and its efficient function is critical for fast muscle fibers.

Pathways

SERCA1 ATPase is a significant component of the calcium signaling and muscle contraction pathways. In the context of muscle contraction the release and reuptake of Ca2+ ions regulated by SERCA1 ATPase are central events. The protein works closely with the ryanodine receptor (RyR) and calsequestrin which also participate in the modulation of intracellular calcium levels. Their interactions ensure precise coordination during muscle contraction and relaxation processes.

Mutations or dysregulation of SERCA1 ATPase can lead to conditions such as Brody disease and certain forms of myopathy. Brody disease is characterized by impaired muscle relaxation which directly relates to the malfunctioning of this Ca2+ ATPase. Additionally the disrupted function of SERCA1 ATPase may also involve interactions with other proteins like the ryanodine receptor which can exacerbate muscle-related symptoms and contribute to the pathophysiology of these disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum (PubMed : 21697544, PubMed : 22961106, PubMed : 25640239, PubMed : 26816378, PubMed : 27923914). Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (PubMed : 21697544, PubMed : 22961106, PubMed : 25640239, PubMed : 26816378).
See full target information Atp2a1
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文献 (21)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8404 PubMed40998795

2025

Injectable therapeutic system incorporating neurogenesis-programmed stem cells concomitantly promoting muscle regeneration treats stress urinary incontinence.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzhuo Fang,Xuan Du,Ranxing Yang,Meng Liu,Ming Yang,Yangwang Jin,Guo Gao,Qiang Fu,Ying Wang

Journal of cell science 130:841-852 PubMed28104817

2017

Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Hofhuis,Kristina Bersch,Ronja Büssenschütt,Marzena Drzymalski,David Liebetanz,Viacheslav O Nikolaev,Stefan Wagner,Lars S Maier,Jutta Gärtner,Lars Klinge,Sven Thoms

Proteomes 3:298-327 PubMed28248273

2015

Concurrent Label-Free Mass Spectrometric Analysis of Dystrophin Isoform Dp427 and the Myofibrosis Marker Collagen in Crude Extracts from Skeletal Muscles.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Murphy,Margit Zweyer,Rustam R Mundegar,Michael Henry,Paula Meleady,Dieter Swandulla,Kay Ohlendieck

Biology 4:397-423 PubMed26067837

2015

Simultaneous Pathoproteomic Evaluation of the Dystrophin-Glycoprotein Complex and Secondary Changes in the mdx-4cv Mouse Model of Duchenne Muscular Dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Murphy,Michael Henry,Paula Meleady,Margit Zweyer,Rustam R Mundegar,Dieter Swandulla,Kay Ohlendieck

PloS one 9:e107753 PubMed25229238

2014

New insights into the relationship between mIGF-1-induced hypertrophy and Ca2+ handling in differentiated satellite cells.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Guarnieri,Caterina Morabito,Silvia Belia,Laura Barberi,Antonio Musarò,Giorgio Fanò-Illic,Maria A Mariggiò

The Journal of clinical investigation 123:1229-40 PubMed23376793

2013

Muscle lipogenesis balances insulin sensitivity and strength through calcium signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Katsuhiko Funai,Haowei Song,Li Yin,Irfan J Lodhi,Xiaochao Wei,Jun Yoshino,Trey Coleman,Clay F Semenkovich

The Journal of physiology 590:6187-97 PubMed22988144

2012

Impaired mitochondrial respiration and decreased fatigue resistance followed by severe muscle weakness in skeletal muscle of mitochondrial DNA mutator mice.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Yamada,Niklas Ivarsson,Andrés Hernández,Andreas Fahlström,Arthur J Cheng,Shi-Jin Zhang,Joseph D Bruton,Brun Ulfhake,Håkan Westerblad

PloS one 7:e44343 PubMed22952960

2012

Brain-derived neurotrophic factor enhances calcium regulatory mechanisms in human airway smooth muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Amard J Abcejo,Venkatachalem Sathish,Dan F Smelter,Bharathi Aravamudan,Michael A Thompson,William R Hartman,Christina M Pabelick,Y S Prakash

The Journal of physiology 590:3575-83 PubMed22687611

2012

Dietary nitrate increases tetanic [Ca2+]i and contractile force in mouse fast-twitch muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés Hernández,Tomas A Schiffer,Niklas Ivarsson,Arthur J Cheng,Joseph D Bruton,Jon O Lundberg,Eddie Weitzberg,Håkan Westerblad

Biochemical and biophysical research communications 420:236-40 PubMed22405774

2012

Phospholamban mutants compete with wild type for SERCA binding in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Simon J Gruber,Suzanne Haydon,David D Thomas
View all publications
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