AB154112

Anti-Septin 8 抗体

Name: Anti-Septin 8 抗体 (ab154112) | Abcam
Brand: Abcam Limited
SKU: AB154112
Price: 76000 JPY
Availability: InStock

Anti-Septin 8 antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal Septin 8 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SEPTIN8 aa 150 to C-terminus.

別名を表示する

KIAA0202, SEPT8, SEPTIN8, Septin-8

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling Septin 8 with ab154112 at 1/200 dilution (green).
Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

Immunofluorescence analysis of DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min labelling SEPT8 protein using ab154112 at a 1/500 dilution (Green).
beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

Unknown

Western blot - Anti-Septin 8 antibody (AB154112)

12% SDS PAGE

All lanes:

Western blot - Anti-Septin 8 antibody (ab154112) at 1/2000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-Septin 8 antibody (AB154112)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Septin 8 antibody (ab154112) at 1/4000 dilution

Lane 1:

Mouse brain tissue extracts at 10 µg

Lane 2:

Rat brain tissue extracts at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human SEPTIN8 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92599

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Septin 8 often referred to as SEPT8 belongs to the septin family of GTPase proteins. It participates in filament formation by polymerizing with other septins. This protein has a molecular weight of around 52 kDa. SEPT8 is expressed in various tissues with significant presence in the brain and heart. Its function depends on cell type and developmental stage exhibiting dynamic patterns of expression which make it integral to cellular processes.

Biological function summary

The protein is involved in maintaining cell structure and polarity. SEPT8 is part of larger septin complexes necessary for correct cytokinesis. It regulates cell division by contributing to the formation of cleavage furrow during mitosis. SEPT8 also influences membrane dynamics and vesicle trafficking showcasing a role in transport within cells.

Pathways

Septin 8 contributes to cytoskeleton organization and vesicle trafficking pathways. It associates with proteins such as actin and myosin playing roles in cell shape changes and intracellular transport. SEPT8 functions alongside other septins in the septin ring structures which are essential for several processes including cell cycle progression and vesicle sorting.

Septin 8 has been linked to neurodegenerative conditions and cancer. Abnormal SEPT8 expression or mutations might lead to Alzheimer's disease due to its impact on neuronal transport systems. SEPT8 is also observed in tumorigenesis interfering with normal cell cycle control. In cancer its interaction with other septins could disrupt normal cell proliferation suggesting it as a potential therapeutic target.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Filament-forming cytoskeletal GTPase (By similarity). May play a role in platelet secretion (PubMed : 15116257). Seems to participate in the process of SNARE complex formation in synaptic vesicles (By similarity).. Isoform 4. Stabilizes BACE1 protein levels and promotes the sorting and accumulation of BACE1 to the recycling or endosomal compartments, modulating the beta-amyloidogenic processing of APP.

See full target information SEPTIN8

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 82:106-122.e9 PubMed34875212

2021

SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity.

Applications

Unspecified application

Species

Unspecified reactive species

Daoud Sheban,Tom Shani,Roey Maor,Alejandro Aguilera-Castrejon,Nofar Mor,Bernardo Oldak,Merav D Shmueli,Avital Eisenberg-Lerner,Jonathan Bayerl,Jakob Hebert,Sergey Viukov,Guoyun Chen,Assaf Kacen,Vladislav Krupalnik,Valeriya Chugaeva,Shadi Tarazi,Alejandra Rodríguez-delaRosa,Mirie Zerbib,Adi Ulman,Solaiman Masarwi,Meital Kupervaser,Yishai Levin,Efrat Shema,Yael David,Noa Novershtern,Jacob H Hanna,Yifat Merbl

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Septin 8 抗体

Anti-Septin 8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

Immunocytochemistry/ Immunofluorescence - Anti-Septin 8 antibody (AB154112)

Western blot - Anti-Septin 8 antibody (AB154112)

Western blot - Anti-Septin 8 antibody (AB154112)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise