Anti-SEPT7 抗体 [EPR12610] (ab175229)
![Western blot - Anti-SEPT7 antibody [EPR12610] (ab175229)](https://www.abcam.co.jp/ps/products/175/ab175229/Images/ab175229-201578-anti-sept7-antibody-epr12610-western-blot.jpg "Western blot - Anti-SEPT7 antibody [EPR12610] (ab175229)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12610] to SEPT7
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SEPT7 antibody [EPR12610]
SEPT7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12610] to SEPT7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment within Human SEPT7. The exact sequence is proprietary.
Database link: Q16181 -
ポジティブ･コントロール
- Jurkat, HeLa, MCF7 and HepG2 whole cell lysate (ab7900); HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12610 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175229の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
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| ICC/IF |
1/50 - 1/100.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 51 kDa. |
|
ICC/IF
1/50 - 1/100. |
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.
ターゲット情報
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機能
Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the septin family. -
細胞内局在
Cytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Cytoplasm > cytoskeleton > cilium axoneme. Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase. Associated with actin stress fibers. - Information by UniProt
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参照データベース
- Entrez Gene: 989 Human
- Entrez Gene: 235072 Mouse
- Entrez Gene: 64551 Rat
- Omim: 603151 Human
- SwissProt: Q16181 Human
- SwissProt: O55131 Mouse
- SwissProt: Q9WVC0 Rat
- Unigene: 191346 Human
see all -
別名
- CDC10 antibody
- CDC10 protein homolog antibody
- CDC3 antibody
see all
画像
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Western blot - Anti-SEPT7 antibody [EPR12610] (ab175229)All lanes : Anti-SEPT7 antibody [EPR12610] (ab175229) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] (ab175229)](https://www.abcam.co.jp/ps/products/175/ab175229/Images/ab175229-201577-anti-sept7-antibody-epr12610-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody [EPR12610] (ab175229)Immunofluorescent analysis of HeLa cells labeling SEPT7 with ab175229 at 1/50 dilution. DAPI nuclear staining (blue).
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![Anti-SEPT7 antibody [EPR12610] (ab175229)](https://www.abcam.co.jp/ps/products/175/ab175229/Images/ab175229-3-benefits-of-recombinant-antibodies.png)
Anti-SEPT7 antibody [EPR12610] (ab175229)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab175229 は 5 報の論文で使用されています。
- Stepan J et al. Hippo-released WWC1 facilitates AMPA receptor regulatory complexes for hippocampal learning. Cell Rep 41:111766 (2022). PubMed: 36476872
- Chen F et al. FIT2 organizes lipid droplet biogenesis with ER tubule-forming proteins and septins. J Cell Biol 220:N/A (2021). PubMed: 33861319
- Collins KB et al. Septin2 mediates podosome maturation and endothelial cell invasion associated with angiogenesis. J Cell Biol 219:N/A (2020). PubMed: 31865373
- Li R et al. Septin 7 mediates high glucose-induced podocyte apoptosis. Biochem Biophys Res Commun 506:522-528 (2018). PubMed: 30361092
- Sinclair LI & Love S Effect of APOE Genotype on Synaptic Proteins in Earlier Adult Life. J Alzheimers Dis 59:1123-1137 (2017). PubMed: 28731446