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AB108981

Anti-SENP1 抗体 [EPR3844]

Anti-SENP1 antibody [EPR3844]

5

(7 Reviews)

|

(43 Publications)

Rabbit Recombinant Monoclonal SENP1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 43 publications.

別名を表示する

Sentrin-specific protease 1, Sentrin/SUMO-specific protease SENP1, SENP1

8 Images
Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)
  • WB

Lab

Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/20000 dilution

All lanes:

U87-MG cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Flow Cytometry (Intracellular) - Anti-SENP1 antibody [EPR3844] (AB108981)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SENP1 antibody [EPR3844] (AB108981)

Intracellular Flow Cytometry analysis of HeLa cells labelling SENP1 with purified ab108981 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP1 antibody [EPR3844] (AB108981)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SENP1 antibody [EPR3844] (AB108981)

Immunohistochemical staining of paraffin embedded human testis with purified ab108981 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (AB108981)
  • ICC/IF

AbReview29250****

Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (AB108981)

Unpurified ab108981 (1/500) staining SENP1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (AB108981)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SENP1 antibody [EPR3844] (AB108981)

Immunofluorescence staining of Jurkat cells with purified ab108981 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108981 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)
  • WB

Unknown

Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)

All lanes:

Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/1000 dilution

Lane 1:

HeLa cell lysates at 10 µg

Lane 2:

HUVEC cell lysates at 10 µg

Lane 3:

Jurkat cell lysates at 10 µg

Lane 4:

Daudi cell lysates at 10 µg

Lane 5:

U87-MG cell lysates at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 73 kDa

false

Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)
  • WB

AbReview48238****

Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)

Blocked with 3% milk for 1 hour at 25°C.

Incubated with the primary antibody for 18 hours at 4°C.

All lanes:

Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/1000 dilution

Lane 1:

COS-1 cell lysate at 20000 Cells

Lane 2:

COS-1 cell lysate transfected with SENP1 at 20000 Cells

Lane 3:

COS-1 cell lysate transfected with SENP1 mutant at 20000 Cells

Secondary

All lanes:

HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 73 kDa

Observed band size: 75 kDa

true

Exposure time: 6min

This image is courtesy of an Abreview submitted by Ragnhild Eskeland

Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)
  • WB

Lab

Western blot - Anti-SENP1 antibody [EPR3844] (AB108981)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SENP1 antibody [EPR3844] (ab108981) at 1/20000 dilution

All lanes:

Daudi cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

関連する標識済み抗体及び組成の異なる製品 (2)

  • HRP

    HRP Anti-SENP1 antibody [EPR3844]

  • Carrier free

    Anti-SENP1 antibody [EPR3844] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3844

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt (Intra), WB, IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/300", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SENP1 also known as SUMO-specific protease 1 is an enzyme involved in the process of sumoylation a post-translational modification mechanism affecting various proteins. This enzyme has an approximate molecular mass of 73 kDa. SENP1 specifically cleaves SUMO (Small Ubiquitin-like Modifier) from target proteins regulating their function and localization. It shows strong expression in tissues with high cellular turnover like the thymus spleen and testis indicating its significant role in cell growth and development processes.
Biological function summary

SENP1 contributes to cellular homeostasis by modifying and controlling protein interactions and functions. This enzyme facilitates the recycling of SUMO proteins and affects transcription factors such as HIF1α which are vital for cellular response to hypoxia. SENP1 interacts closely with SUMO E3 ligases in complexes coordinating the addition or removal of SUMO groups from target proteins. Its activity is essential for maintaining the balance between protein sumoylation and desumoylation which impacts numerous cellular processes including cell cycle progression and stress response.

Pathways

SENP1 modulates key regulatory pathways related to its sumoylation mechanism. Major pathways where SENP1 plays a role include the HIF-1 signaling pathway where it affects the stability and activity of HIF1α under low oxygen conditions and the p53 signaling pathway influencing cell cycle and apoptosis. SENP1's interactivity with proteins like Ubc9 a SUMO-conjugating enzyme and PIAS3 a SUMO E3 ligase indicates its broad involvement and regulation within these pathways emphasizing its impact on maintaining cellular function and adaptation.

SENP1 associates with certain cancers and neurodegenerative disorders due to its regulatory influence on protein stability and gene expression. Overexpression of SENP1 is often observed in prostate cancer where it desumoylates HIF1α enhancing its transcriptional activity and promoting tumorigenesis. Additionally alterations in SENP1 function might connect to neurodegenerative diseases like Alzheimer's where protein turnover and cellular stress responses are disrupted. Through these disorders SENP1 connects with proteins such as AR (androgen receptor) in cancer contexts and Tau in Alzheimer's underlying its role in disease pathology.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Protease that catalyzes two essential functions in the SUMO pathway (PubMed : 10652325, PubMed : 15199155, PubMed : 15487983, PubMed : 16253240, PubMed : 16553580, PubMed : 21829689, PubMed : 21965678, PubMed : 23160374, PubMed : 24943844, PubMed : 25406032, PubMed : 29506078, PubMed : 34048572, PubMed : 37257451). The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins (PubMed : 15487983). The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein (PubMed : 15199155, PubMed : 16253240, PubMed : 21829689, PubMed : 21965678, PubMed : 23160374, PubMed : 24943844, PubMed : 25406032, PubMed : 29506078, PubMed : 34048572, PubMed : 37257451). Deconjugates SUMO1 from HIPK2 (PubMed : 16253240). Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity (PubMed : 15199155, PubMed : 21829689). Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity (PubMed : 23160374). Deconjugates SUMO2 from MTA1 (PubMed : 21965678). Inhibits N(6)-methyladenosine (m6A) RNA methylation by mediating SUMO1 deconjugation from METTL3 and ALKBH5 : METTL3 inhibits the m6A RNA methyltransferase activity, while ALKBH5 desumoylation promotes m6A demethylation (PubMed : 29506078, PubMed : 34048572, PubMed : 37257451). Desumoylates CCAR2 which decreases its interaction with SIRT1 (PubMed : 25406032). Deconjugates SUMO1 from GPS2 (PubMed : 24943844).
See full target information SENP1
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文献 (43)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5688 PubMed37709794

2023

N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization.

Applications

Unspecified application

Species

Unspecified reactive species

Weiji Weng,Xiaokun Gu,Yang Yang,Qiao Zhang,Qi Deng,Jie Zhou,Jinke Cheng,Michael X Zhu,Junfeng Feng,Ou Huang,Yong Li

Nature communications 13:7153 PubMed36414671

2022

SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Lingjie Yan,Tao Zhang,Kai Wang,Zezhao Chen,Yuanxin Yang,Bing Shan,Qi Sun,Mengmeng Zhang,Yichi Zhang,Yedan Zhong,Nan Liu,Jinyang Gu,Daichao Xu

Oncogenesis 11:65 PubMed36284084

2022

The SUMO protease SENP1 promotes aggressive behaviors of high HIF2α expressing renal cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Moon Hee Lee,Kyung Sung,David Beebe,Wei Huang,Dan Shapiro,Shigeki Miyamoto,E Jason Abel

Oxidative medicine and cellular longevity 2022:8002566 PubMed35707278

2022

Ginkgolic acid improves bleomycin-induced pulmonary fibrosis by inhibiting SMAD4 SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Yu,Xiyun Bian,Chunyan Zhang,Zhouying Wu,Na Huang,Jie Yang,Wen Jin,Zongqi Feng,Dongfang Li,Xue Huo,Ting Wu,Zhongmin Jiang,Xiaozhi Liu,Dejun Sun

International journal of biological sciences 18:2186-2201 PubMed35342335

2022

SENP1 promotes triple-negative breast cancer invasion and metastasis via enhancing CSN5 transcription mediated by GATA1 deSUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yongchang Gao,Rongrong Wang,Jianjing Liu,Ke Zhao,Xiaolong Qian,Xianghui He,Hong Liu

Frontiers in cell and developmental biology 9:789348 PubMed35186948

2022

TCR-Induced Tyrosine Phosphorylation at Tyr270 of SUMO Protease SENP1 by Lck Modulates SENP1 Enzyme Activity and Specificity.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Yi Li,Haohua Cen,Bei-Ni Gong,Siqi Mai,Qi-Long Wang,Sisi Mou,Yingqiu Li

Cell death & disease 12:1067 PubMed34753901

2021

Knockdown of ubiquitin-like modifier-activating enzyme 2 promotes apoptosis of clear cell renal cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guoxi Zhang,Junrong Zou,Jinglin Shi,Biao Qian,Kaiyang Qiu,Quanliang Liu,Tianpeng Xie,Zhihua He,Hui Xu,Yunfeng Liao,Yuting Wu,Yanmin Li,Guancheng Xiao,Yuanhu Yuan,Rihai Xiao,Gengqing Wu,Xiaofeng Zou

Journal of cell science 134: PubMed34313310

2021

SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Yu,Qiaoyu Lin,Chao Huang,Boyan Zhang,Ying Wang,Qing Jiang,Chuanmao Zhang,Jing Yi

The FEBS journal 288:6447-6464 PubMed34089566

2021

The interplay between SUMOylation and phosphorylation of PKCδ facilitates oxidative stress-induced apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Siman Gao,Xiangteng Zhao,Lin Hou,Ruining Ma,Jie Zhou,Michael X Zhu,Si-Jian Pan,Yong Li

Cell death & disease 12:341 PubMed33795649

2021

SENP1-mediated deSUMOylation of JAK2 regulates its kinase activity and platinum drug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Ruiqin Wu,Mingo M H Yung,Jing Sun,Zhuqing Li,Hai Yang,Yi Zhang,Stephanie S Liu,Annie N Y Cheung,Hextan Y S Ngan,John C Braisted,Wei Zheng,Huiqiang Wei,Yingtang Gao,Peter Nemes,Huadong Pei,David W Chan,Yiliang Li,Wenge Zhu
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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