Anti-Senataxin 抗体 [EPR24137-74] (ab300439)
![Western blot - Anti-Senataxin antibody [EPR24137-74] (AB300439)](https://www.abcam.co.jp/ps/products/300/ab300439/Images/AB300439-487168-anti-Senataxin-antibody-EPR2413774-WB-HeLa.jpg "Western blot - Anti-Senataxin antibody [EPR24137-74] (AB300439)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24137-74] to Senataxin
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Senataxin antibody [EPR24137-74]
Senataxin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24137-74] to Senataxin -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details
適用なし: ChIP,ICC/IF,IHC-Fr,IHC-P or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa (human cervical adenocarcinoma epithelial cell), SH-SY5Y (human neuroblastoma epithelial cell), SK-N-BE(2) (human neuroblastoma neuroblast), IMR-32 (human neuroblastoma neuroblast). Negative control: HeLa transfected with scrambled siRNA control. Flow Cyt (Intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24137-74 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300439の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000.
|
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| Flow Cyt (Intra) |
1/50.
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| 特記事項 |
|---|
|
WB
1/1000. |
|
Flow Cyt (Intra)
1/50. |
ターゲット情報
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機能
Probable helicase, which may be involved in RNA maturation (By similarity). Involved in DNA double-strand breaks damage response generated by oxidative stress. -
組織特異性
Highly expressed in skeletal muscle. Expressed in heart, fibroblast, placenta and liver. Weakly expressed in brain and lung. Expressed in the cortex of the kidney (highly expressed in tubular epithelial cells but low expression in the glomerulus). -
関連疾患
Defects in SETX are the cause of spinocerebellar ataxia autosomal recessive type 1 (SCAR1) [MIM:606002]; also known as ataxia-ocular apraxia 2. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR1 is an autosomal recessive form associated with peripheral neuropathy and elevated serum alpha-fetoprotein, immunoglobulins and, less commonly, creatine kinase levels. Some SCAR1 patients manifest oculomotor apraxia.
Defects in SETX are a cause of amyotrophic lateral sclerosis type 4 (ALS4) [MIM:602433]. ALS4 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. ALS4 is a childhood- or adolescent-onset form characterized by slow disease progression and the sparing of bulbar and respiratory muscles. -
配列類似性
Belongs to the DNA2/NAM7 helicase family. -
細胞内局在
Nucleus > nucleoplasm. Nucleus > nucleolus. Cytoplasm. May be detected in the nucleolus only in cycling cells (By similarity). Most abundant in the nucleus. Detected in granules. Colocalized in cycling cells with FBL in the nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 23064 Human
- Omim: 608465 Human
- SwissProt: Q7Z333 Human
- Unigene: 460317 Human
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別名
- ALS4 antibody
- Amyotrophic lateral sclerosis 4 protein antibody
- AOA2 antibody
see all
画像
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Western blot - Anti-Senataxin antibody [EPR24137-74] (AB300439)All lanes : Anti-Senataxin antibody [EPR24137-74] (ab300439) at 1/1000 dilution
Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting SETX, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 300 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsBlocking / Diluting buffer and concentration:5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:17562789).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. -
![Western blot - Anti-Senataxin antibody [EPR24137-74] (AB300439)](https://www.abcam.co.jp/ps/products/300/ab300439/Images/AB300439-487170-anti-Senataxin-antibody-EPR2413774-WB-shsy5y.jpg)
Western blot - Anti-Senataxin antibody [EPR24137-74] (AB300439)Anti-Senataxin antibody [EPR24137-74] (ab300439) at 1/1000 dilution + SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 300 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking / Diluting buffer and concentration:5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:17562789).
The lysate was freshly made and used for Western blotting immediately to minimize protein degradation. -
![Western blot - Anti-Senataxin antibody [EPR24137-74] (AB300439)](https://www.abcam.co.jp/ps/products/300/ab300439/Images/AB300439-487169-anti-Senataxin-antibody-EPR2413774-WB-human-neuroblastoma.jpg)
Western blot - Anti-Senataxin antibody [EPR24137-74] (AB300439)All lanes : Anti-Senataxin antibody [EPR24137-74] (ab300439) at 1/1000 dilution
Lane 1 : SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate
Lane 2 : IMR-32 (human neuroblastoma neuroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 300 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking / Diluting buffer and concentration:5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:17562789).
The bands beneath the target band are likely to be degraded target fragments.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. -
![Flow Cytometry (Intracellular) - Anti-Senataxin antibody [EPR24137-74] (AB300439)](https://www.abcam.co.jp/ps/products/300/ab300439/Images/AB300439-487167-anti-Senataxin-antibody-EPR2413774-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-Senataxin antibody [EPR24137-74] (AB300439)Flow Cytometry (Intracellular) analysis of HeLa (human cervix adenocarcinoma epithelial cells) labeling Senataxin (red) with ab300439 at a 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, (ab150081)) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730) Isotype control. Blue - (unlabeled control) - Cells without incubation with primary and secondary antibodies.
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![Anti-Senataxin antibody [EPR24137-74] (AB300439)](https://www.abcam.co.jp/ps/products/300/ab300439/Images/AB300439-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Senataxin antibody [EPR24137-74] (AB300439)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300439 は論文での使用が確認できていません。