AB23578

Anti-Semaphorin 7a 抗体

Anti-Semaphorin 7a antibody

(2 Reviews)

(25 Publications)

Rabbit Polyclonal Semaphorin 7a antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 25 publications.

別名を表示する

CD108, Cd108, Semal, Semk1, Sema7a, Semaphorin-7A, Semaphorin-K1, Semaphorin-L, Sema K1, Sema L

2 Images

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Immunoprecipitation - Anti-Semaphorin 7a antibody (AB23578)

Semaphorin 7a was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Semaphorin 7a and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab23578.
Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band : 75kDa : Semaphorin 7a.

All lanes:

Immunoprecipitation - Anti-Semaphorin 7a antibody (ab23578)

Predicted band size: 75 kDa

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Lab

Western blot - Anti-Semaphorin 7a antibody (AB23578)

Gel type : MOPS

Blocking buffer : 2% BSA block

All lanes:

Western blot - Anti-Semaphorin 7a antibody (ab23578) at 1 µg/mL

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse cerebellum tissue lysate at 10 µg

Lane 3:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa,90 kDa

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Exposure time: 8min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab23578 detects Semaphorin 7a protein at 75 kDa by WB. Semaphorin 7a is a protein that is tethered to the cell membrane via a GPI-anchor and is also glycosylated, therefore it is not surprising that in WB we see some additional bands at ~50 and 100 kDa which are likely to be cleavage fragments and glycosylated protein respectively. Preincubation with the immunizing peptide ab30844 is successful in blocking antibody staining.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Negative control: HEK cells

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Semaphorin 7a also known as SEMA7A or CD108 is a glycoprotein that has a molecular mass of approximately 80 kDa. This target plays a role in axonal guidance and immune response regulation. It is a member of the semaphorin family which generally acts as signaling molecules. Semaphorin 7a is expressed in various tissues including the immune system nervous system and some epithelial cells. Its expression is especially significant in T-cells and is involved in modulating cell adhesion and migration.

Biological function summary

Semaphorin 7a is implicated in diverse processes such as immune system modulation and nervous system development. It functions as part of a complex involving integrins which mediate cell-to-cell communication and interaction with the extracellular matrix. This protein can bridge the immune and neural signaling pathways indicating its multifunctional nature within organisms. Its interaction with β1 integrins enhances T-cell activation and cytokine production which is important for immune response.

Pathways

Semaphorin 7a integrates into the MAPK and PI3K signaling pathways. These pathways are essential for cell survival migration and apoptosis. In the MAPK pathway Semaphorin 7a impacts downstream signaling through its interaction with ERK proteins. Its role in the PI3K pathway connects it to AKT proteins influencing cell growth and metabolic processes. These connections show its versatility and critical role in signal transduction linked to cellular behaviors.

Semaphorin 7a has been associated with multiple sclerosis and melanoma. In multiple sclerosis altered expression of this protein can affect immune response and neural repair processes. Its connection to melanoma is linked with its role in regulating cell motility and adhesion impacting cancer progression and metastasis. Semaphorin 7a's interactions with proteins like Melanocortin 1 Receptor (MC1R) in melanoma provide insights into therapeutic targets for disease interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of pro-inflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.

See full target information Sema7a

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文献 (25)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 20:5039-5057 PubMed40264818

2025

Heme Oxygenase-1 Modulates Macrophage Polarization Through Endothelial Exosomal miR-184-3p and Reduces Sepsis-Induce Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Chen,Yuan Zhang,Jinkun Chen,Shuan Dong,Xiaoyang Wu,Ya Wu,Zhuo Du,Yibo Yang,Lirong Gong,Jianbo Yu

Frontiers in immunology 14:1251026 PubMed38094294

2023

Semaphorin 7A is protective during inflammatory peritonitis through integrin receptor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Körner,David Köhler,Mariella Schneider,Judith M Roth,Tiago F Granja,Claudia Eggstein,Valbona Mirakaj,Peter Rosenberger

Frontiers in cell and developmental biology 11:1261038 PubMed37941899

2023

Dynamic regulation of semaphorin 7A and adhesion receptors in ovarian follicle remodeling and ovulation.

Applications

Unspecified application

Species

Unspecified reactive species

Alaknanda Emery,Kylie R Dunning,Doan T Dinh,Lisa K Akison,Rebecca L Robker,Darryl L Russell

Respiratory research 24:12 PubMed36631871

2023

Impaired cell-cell communication and axon guidance because of pulmonary hypoperfusion during postnatal alveolar development.

Applications

Unspecified application

Species

Unspecified reactive species

Debao Li,Jing Wang,Yuan Fang,Yuqing Hu,Yingying Xiao,Qing Cui,Chuan Jiang,Sijuan Sun,Hao Chen,Lincai Ye,Qi Sun

Neuron 111:190-201.e8 PubMed36384142

2022

Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development.

Applications

Unspecified application

Species

Unspecified reactive species

Yixun Su,Xiaorui Wang,Yujian Yang,Liang Chen,Wenlong Xia,Kimberly K Hoi,Hui Li,Qi Wang,Guangdan Yu,Xiaoying Chen,Shouyu Wang,Yuxin Wang,Lan Xiao,Alexei Verkhratsky,Stephen P J Fancy,Chenju Yi,Jianqin Niu

Nature communications 13:6040 PubMed36229455

2022

Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system.

Applications

Unspecified application

Species

Unspecified reactive species

Noemie Vilallongue,Julia Schaeffer,Anne-Marie Hesse,Céline Delpech,Béatrice Blot,Antoine Paccard,Elise Plissonnier,Blandine Excoffier,Yohann Couté,Stephane Belin,Homaira Nawabi

Oxidative medicine and cellular longevity 2021:8899274 PubMed34007409

2021

Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yunen Liu,Changci Tong,Peifang Cong,Ying Liu,Xiuyun Shi,Lin Shi,Shun Mao,Yan Zhao,Hongxu Jin,Mingxiao Hou

Nature communications 11:1315 PubMed32161256

2020

Red blood cell-derived semaphorin 7A promotes thrombo-inflammation in myocardial ischemia-reperfusion injury through platelet GPIb.

Applications

Unspecified application

Species

Unspecified reactive species

David Köhler,Tiago Granja,Julia Volz,Michael Koeppen,Harald F Langer,Georg Hansmann,Ekaterina Legchenko,Tobias Geisler,Tamam Bakchoul,Claudia Eggstein,Helene A Häberle,Bernhard Nieswandt,Peter Rosenberger

Cell biochemistry and function 38:58-65 PubMed31732974

2019

LncRNA LOXL1-AS1/miR-28-5p/SEMA7A axis facilitates pancreatic cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yongcun Liu,Cheng Guo,Feng Li,Liping Wu

Frontiers in physiology 9:1718 PubMed30555351

2018

Semaphorin 7A Promotes VEGFA/VEGFR2-Mediated Angiogenesis and Intraplaque Neovascularization in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhong Hu,Yifei Liu,Tao You,Li Zhu

View all publications

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Abcam product promise

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Anti-Semaphorin 7a 抗体

Anti-Semaphorin 7a antibody

Immunoprecipitation - Anti-Semaphorin 7a antibody (AB23578)

Western blot - Anti-Semaphorin 7a antibody (AB23578)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (25)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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