Anti-Semaphorin 3E 抗体 (ab112886)
Key features and details
- Goat polyclonal to Semaphorin 3E
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Semaphorin 3E antibody -
製品の詳細
Goat polyclonal to Semaphorin 3E -
由来種
Goat -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig
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免疫原
Synthetic peptide:
NGGRYGTTKDYPDD
by a Cysteine residue linker, corresponding to internal sequence amino acids 387-400 of Human Semaphorin 3E (NP_036563.1). -
ポジティブ･コントロール
- Human Prostate tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab112886 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112886の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Sema domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 9723 Human
- Entrez Gene: 20349 Mouse
- Entrez Gene: 296789 Rat
- Omim: 608166 Human
- SwissProt: O15041 Human
- SwissProt: P70275 Mouse
- Unigene: 528721 Human
- Unigene: 134093 Mouse
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別名
- Coll 5 antibody
- Coll5 antibody
- KIAA0331 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 3E antibody (ab112886)ab112886 at 4µg/ml showing membranous staining of Semaphorin 3E in the secretory cells of Paraffin-embedded Human Prostate tissue by Immunohistochemistry. Detection utilised HRP staining.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab112886 は 2 報の論文で使用されています。
- Wu JH et al. Semaphorin-3E attenuates neointimal formation via suppressing VSMCs migration and proliferation. Cardiovasc Res 113:1763-1775 (2017). PubMed: 29016743
- Mazzotta C et al. Plexin-D1/Semaphorin 3E pathway may contribute to dysregulation of vascular tone control and defective angiogenesis in systemic sclerosis. Arthritis Res Ther 17:221 (2015). IHC-P ; Human . PubMed: 26292963