Anti-SELH 抗体 (ab151023)
Key features and details
- Rabbit polyclonal to SELH
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SELH antibody -
製品の詳細
Rabbit polyclonal to SELH -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster
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免疫原
Recombinant fragment, corresponding to amino acids 68-122 of Human SELH (Q8IZQ5).
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ポジティブ･コントロール
- ICC: MCF7 whole cell; IHC-P: Human tonsil, cerebral cortex, prostate and small intestine tissue.
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特記事項
The antibody solution should be gently mixed before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151023の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 特記事項 |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
ターゲット情報
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機能
May be involved in a redox-related process. -
配列類似性
Belongs to the SelWTH family. - Information by UniProt
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参照データベース
- Entrez Gene: 280636 Human
- Entrez Gene: 72657 Mouse
- Entrez Gene: 502642 Rat
- Omim: 607914 Human
- SwissProt: Q8IZQ5 Human
- SwissProt: Q3UQA7 Mouse
- Unigene: 655373 Human
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別名
- C11orf31 antibody
- Chromosome 11 open reading frame 31 antibody
- Selenoprotein H antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-SELH antibody (ab151023)
Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) whole cells labelling SELH at the nucleus and nucleoli with ab151023 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELH antibody (ab151023)Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling SELH at the nucleus of non-germinal center cells with ab151023 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELH antibody (ab151023)Immunohistochemical analysis of paraffin embedded human cerebral cortex tissue labeling SELH at the nucleus neurons with ab151023 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELH antibody (ab151023)Immunohistochemical analysis of paraffin embedded human prostate tissue labeling SELH at the nucleus of glandular cells with ab151023 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SELH antibody (ab151023)Immunohistochemical analysis of paraffin embedded human small intestine tissue labeling SELH at the nucleus of lymphoid cells with ab151023 antibody at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab151023 は 3 報の論文で使用されています。
- Schwarz M et al. Copper interferes with selenoprotein synthesis and activity. Redox Biol 37:101746 (2020). PubMed: 33059313
- Schwarz M et al. Crosstalk of Nrf2 with the Trace Elements Selenium, Iron, Zinc, and Copper. Nutrients 11:N/A (2019). PubMed: 31491970
- Hiller F et al. Differential acute effects of selenomethionine and sodium selenite on the severity of colitis. Nutrients 7:2687-706 (2015). PubMed: 25867950