Anti-Selenophosphate synthetase 2 抗体 (ab153878)
Key features and details
- Rabbit polyclonal to Selenophosphate synthetase 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Selenophosphate synthetase 2 antibody
Selenophosphate synthetase 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Selenophosphate synthetase 2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Dog, Pig, Monkey
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免疫原
Recombinant fragment, corresponding to a region within amino acids 116-429 of Human Selenophosphate synthetase 2 (UniProt: Q99611).
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ポジティブ･コントロール
- A431, H1299, HeLa and HepG2 whole cell lysates; A431 cells; Human OVCA tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153878の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Synthesizes selenophosphate from selenide and ATP. -
配列類似性
Belongs to the selenophosphate synthase 1 family. Class I subfamily. - Information by UniProt
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参照データベース
- Entrez Gene: 22928 Human
- Entrez Gene: 100037302 Pig
- Omim: 606218 Human
- SwissProt: Q99611 Human
- SwissProt: A1YIZ1 Pig
- Unigene: 118725 Human
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別名
- Selenide antibody
- Selenide, water dikinase 2 antibody
- Selenium donor protein 2 antibody
see all
画像
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Western blot - Anti-Selenophosphate synthetase 2 antibody (ab153878)Anti-Selenophosphate synthetase 2 antibody (ab153878) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Selenophosphate synthetase 2 antibody (ab153878)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells, labeling Selenophosphate synthetase 2 with ab153878 at 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Selenophosphate synthetase 2 antibody (ab153878)Immunohistochemical analysis of paraffin-embedded Human OVCA tissue, labeling Selenophosphate synthetase 2 with ab153878 at 1/100 dilution
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab153878 は 1 報の論文で使用されています。
- Chen J et al. Effects of Selenomethionine on Cell Viability, Selenoprotein Expression and Antioxidant Function in Porcine Mammary Epithelial Cells. Front Nutr 8:665855 (2021). PubMed: 34381803