AB78298

Anti-SEL1L 抗体

Name: Anti-SEL1L 抗体 (ab78298) | Abcam
Brand: Abcam Limited
SKU: AB78298
Price: 55000 JPY
Availability: InStock
Rating: 3 (3 reviews)

Anti-SEL1L antibody

(3 Reviews)

(34 Publications)

Rabbit Polyclonal SEL1L antibody. Suitable for WB and reacts with Human samples. Cited in 34 publications.

別名を表示する

TSA305, UNQ128/PRO1063, SEL1L, Protein sel-1 homolog 1, Suppressor of lin-12-like protein 1, Sel-1L

1 Images

Western blot - Anti-SEL1L antibody (AB78298)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab78298 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID : 18502753).

All lanes:

Western blot - Anti-SEL1L antibody (ab78298) at 1 µg/mL

All lanes:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 89 kDa

Observed band size: 100 kDa

true

Exposure time: 4min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753).</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The SEL1L protein also known as Suppressor/Enhancer of Lin-12-like protein 1 has a molecular mass of approximately 87 kDa. This protein is an important component in various cellular processes. SEL1L exhibits broad expression across different tissues including the pancreas liver and muscle tissues. It interacts with other proteins in the endoplasmic reticulum (ER) to assist in maintaining cellular homeostasis. Through these interactions SEL1L participates directly in managing protein folding and degradation processes.

Biological function summary

The SEL1L protein is critically involved in the ER-associated degradation (ERAD) pathway where it works as part of a complex with other proteins like HRD1. This role allows SEL1L to contribute to the removal of misfolded or damaged proteins from the ER preventing cellular toxicity and dysfunction. In the context of protein quality control SEL1L ensures that only correctly folded proteins proceed to their functional destinations thereby maintaining cellular environment stability and function.

Pathways

SEL1L integrates into the unfolded protein response (UPR) and ERAD processes. The function of SEL1L within these pathways links it to key proteins such as HRD1 and BiP facilitating a critical response mechanism when unprocessed or misfolded proteins accumulate within the ER. By participating in these pathways SEL1L helps in regulating cellular stress responses and preserving protein homeostasis contributing to the proper function of the secretory pathway and cellular stress adaptation.

SEL1L has been associated with conditions such as diabetes and pancreatic cancer. Dysfunction or altered expression of SEL1L can disrupt the regulation of protein homeostasis contributing to misfolded protein accumulation and subsequent cellular stress. In diabetes for example SEL1L interacts with proteins such as XBP1 impacting insulin synthesis and secretion. In pancreatic cancer changes in SEL1L expression are linked with cellular changes and responses within the tumor microenvironment. Understanding SEL1L's roles helps in deciphering the pathology of these disorders and may guide therapeutic strategies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays a role in the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed : 16186509, PubMed : 29997207, PubMed : 37943610, PubMed : 37943617). Enhances SYVN1 stability. Plays a role in LPL maturation and secretion. Required for normal differentiation of the pancreas epithelium, and for normal exocrine function and survival of pancreatic cells. May play a role in Notch signaling.

See full target information SEL1L

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文献 (34)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9244 PubMed39455574

2024

Regulation of hepatic inclusions and fibrinogen biogenesis by SEL1L-HRD1 ERAD.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenfeng Song,Pattaraporn Thepsuwan,Woosuk Steve Hur,Mauricio Torres,Shuangcheng Alivia Wu,Xiaoqiong Wei,Nusrat Jahan Tushi,Juncheng Wei,Francesca Ferraresso,Adrienne W Paton,James C Paton,Ze Zheng,Kezhong Zhang,Deyu Fang,Christian J Kastrup,Sunil Jaiman,Matthew James Flick,Shengyi Sun

Cellular & molecular immunology 21:1441-1458 PubMed39438692

2024

Ubiquitin regulatory X (UBX) domain-containing protein 6 is essential for autophagy induction and inflammation control in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Young Jae Kim,Sung-Gwon Lee,So Young Park,Sang Min Jeon,Soo In Kim,Kyung Tae Kim,Taylor Roh,Sang-Hee Lee,Min Joung Lee,Jinyoung Lee,Hyeon Ji Kim,So Eui Lee,Jin Kyung Kim,Jun Young Heo,In Soo Kim,Chungoo Park,Seungwha Paik,Eun-Kyeong Jo

Cell reports 43:114156 PubMed38687642

2024

ER-associated degradation adapter Sel1L is required for CD8 T cell function and memory formation following acute viral infection.

Applications

Unspecified application

Species

Unspecified reactive species

Luis O Correa-Medero,Shayna E Jankowski,Hanna S Hong,Nicholas D Armas,Aditi I Vijendra,Mack B Reynolds,Garrett M Fogo,Dominik Awad,Alexander T Dils,Kantaro A Inoki,Reid G Williams,Annabelle M Ye,Nadezhda Svezhova,Francisco Gomez-Rivera,Kathleen L Collins,Mary X O'Riordan,Thomas H Sanderson,Costas A Lyssiotis,Shannon A Carty

Nature communications 15:1531 PubMed38378719

2024

Genome-wide screens identify SEL1L as an intracellular rheostat controlling collagen turnover.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Podolsky,Benjamin Kheyfets,Monika Pandey,Afaq H Beigh,Christopher D Yang,Carlos O Lizama,Ritwik Datta,Liangguang L Lin,Zhihong Wang,Paul J Wolters,Michael T McManus,Ling Qi,Kamran Atabai

Cellular and molecular gastroenterology and hepatology 17:1007-1024 PubMed38336172

2024

Multiple Genes Core to ERAD, Macroautophagy and Lysosomal Degradation Pathways Participate in the Proteostasis Response in α1-Antitrypsin Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Francesca Moretti,Tunda Hidvegi,Sanja Sviben,James A J Fitzpatrick,Hemalatha Sundaramoorthi,Stephen C Pak,Gary A Silverman,Britta Knapp,Ireos Filipuzzi,John Alford,John Reece-Hoyes,Florian Nigsch,Leon O Murphy,Beat Nyfeler,David H Perlmutter

The Journal of clinical investigation 134: PubMed37943617

2024

Biallelic Cys141Tyr variant of SEL1L is associated with neurodevelopmental disorders, agammaglobulinemia, and premature death.

Applications

Unspecified application

Species

Unspecified reactive species

Denisa Weis,Liangguang L Lin,Huilun H Wang,Zexin Jason Li,Katarina Kusikova,Peter Ciznar,Hermann M Wolf,Alexander Leiss-Piller,Zhihong Wang,Xiaoqiong Wei,Serge Weis,Katarina Skalicka,Gabriela Hrckova,Lubos Danisovic,Andrea Soltysova,Tingxuan T Yang,René Günther Feichtinger,Johannes A Mayr,Ling Qi

Molecular and cellular biochemistry : PubMed38145449

2023

HRD1 reduction promotes cholesterol-induced vascular smooth muscle cell phenotypic change via endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Linli Wang,Zhitao Ren,Lin Wu,Ximei Zhang,Min Wang,Haiming Niu,Xuemin He,Heting Wang,Yanming Chen,Guojun Shi,Xiaoxian Qian

Nature communications 14:3132 PubMed37253728

2023

The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangcheng Alivia Wu,Chenchen Shen,Xiaoqiong Wei,Xiawei Zhang,Siwen Wang,Xinxin Chen,Mauricio Torres,You Lu,Liangguang Leo Lin,Huilun Helen Wang,Allen H Hunter,Deyu Fang,Shengyi Sun,Magdalena I Ivanova,Yi Lin,Ling Qi

Nature cell biology 25:726-739 PubMed37142791

2023

SEL1L-HRD1 endoplasmic reticulum-associated degradation controls STING-mediated innate immunity by limiting the size of the activable STING pool.

Applications

Unspecified application

Species

Unspecified reactive species

Yewei Ji,Yuan Luo,Yating Wu,Yao Sun,Lianfeng Zhao,Zhen Xue,Mengqi Sun,Xiaoqiong Wei,Zinan He,Shuangcheng Alivia Wu,Liangguang Leo Lin,You Lu,Lei Chang,Fei Chen,Siyu Chen,Wei Qian,Xiaoxi Xu,Shengnuo Chen,Dongli Pan,Zhangsen Zhou,Sheng Xia,Chih-Chi Andrew Hu,Tingbo Liang,Ling Qi

Proceedings of the National Academy of Sciences of the United States of America 120:e2212644120 PubMed36595688

2023

Hepatic SEL1L-HRD1 ER-associated degradation regulates systemic iron homeostasis via ceruloplasmin.

Applications

Unspecified application

Species

Unspecified reactive species

Pattaraporn Thepsuwan,Asmita Bhattacharya,Zhenfeng Song,Stephen Hippleheuser,Shaobin Feng,Xiaoqiong Wei,Nupur K Das,Mariana Sierra,Juncheng Wei,Deyu Fang,Yu-Ming M Huang,Kezhong Zhang,Yatrik M Shah,Shengyi Sun

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SEL1L 抗体

Anti-SEL1L antibody

Western blot - Anti-SEL1L antibody (AB78298)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (34)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Abcam product promise