Anti-Securin 抗体 (ab26273)
Key features and details
- Rabbit polyclonal to Securin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Securin antibody
Securin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Securin -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Securin.
Immunogen の所有権に関して -
ポジティブ･コントロール
- WB: Wild-type HEK293T cell lysate. Daudi and HEK293 cell lysates. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab26273の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
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| ICC/IF | (1) |
1/100.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa). |
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ICC/IF
1/100. |
ターゲット情報
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機能
Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. -
組織特異性
Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer. -
配列類似性
Belongs to the securin family. -
発生段階
Low level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded. -
ドメイン
The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue. -
翻訳後修飾
Phosphorylated at Ser-165 by CDK1 during mitosis.
Phosphorylated in vitro by ds-DNA kinase.
Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9232 Human
- Entrez Gene: 30939 Mouse
- Entrez Gene: 64193 Rat
- Omim: 604147 Human
- SwissProt: O95997 Human
- SwissProt: Q6IAL9 Human
- SwissProt: Q3Y5K4 Mouse
- SwissProt: Q5SRU0 Mouse
see all -
別名
- AW555095 antibody
- C87862 antibody
- Cut2 antibody
see all
画像
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Western blot - Anti-Securin antibody (ab26273)All lanes : Anti-Securin antibody (ab26273) at 1 µg/ml
Lane 1 : HEK293 whole cell lysate with Milk
Lane 2 : Daudi whole cell lysate with Milk
Lysates/proteins at 20 µg per lane.
Blocking peptides at 3 % per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Loading control: GAPDH (ab8245), secondary goat anti-Mouse IgG H&L (IRDye® 680CW) at 1:10,000.
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Immunocytochemistry/ Immunofluorescence - Anti-Securin antibody (ab26273)ab26273 staining Securin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab26273 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunocytochemistry/ Immunofluorescence - Anti-Securin antibody (ab26273)This image is courtesy of Kirk McManusHeLa cells stained with ab26273 (1/100 dilution). A clear nuclear signal in interphase HeLa cells can be seen. -
Western blot - Anti-Securin antibody (ab26273)This image is courtesy of an abreview submitted by Andrew HorwitzAll lanes : Anti-Securin antibody (ab26273) at 1/500 dilution
Lane 1 : HEK293 human cell lysate
Lane 2 : MPRO mouse cell lysate
Lane 3 : S. Cerevisiae lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Polyclonal Goat anti Rabbit at 1/10000 dilution
Predicted band size: 22 kDa
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Western blot - Anti-Securin antibody (ab26273)Anti-Securin antibody (ab26273) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 15 minutes
ab26273 detects a clean 26 kDa band in HEK293 lysate. We would recommend loading 20-40ug of protein when using this antibody. ab26273 has also been tested in mouse lysates and in our hands detects a 70 kDa band which we cannot explain.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab26273 は 5 報の論文で使用されています。
- Xiong X et al. Oocyte-Specific Knockout of Histone Lysine Demethylase KDM2a Compromises Fertility by Blocking the Development of Follicles and Oocytes. Int J Mol Sci 23:N/A (2022). PubMed: 36233308
- Di Domenico M et al. Specificity and Redundancy of Profilin 1 and 2 Function in Brain Development and Neuronal Structure. Cells 10:N/A (2021). PubMed: 34571959
- Lin X et al. PTTG1 is involved in TNF-a-related hepatocellular carcinoma via the induction of c-myc. Cancer Med 8:5702-5715 (2019). PubMed: 31385458
- Lee SB et al. Parkin Regulates Mitosis and Genomic Stability through Cdc20/Cdh1. Mol Cell 60:21-34 (2015). IP . PubMed: 26387737
- Lubka-Pathak M et al. Altered expression of securin (Pttg1) and serpina3n in the auditory system of hearing-impaired Tff3-deficient mice. Cell Mol Life Sci 68:2739-49 (2011). PubMed: 21076990