Anti-Secretogranin V 抗体 (ab22699)
Key features and details
- Rabbit polyclonal to Secretogranin V
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Secretogranin V antibody
Secretogranin V 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Secretogranin V -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Chicken, Xenopus laevis, Zebrafish
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免疫原
Synthetic peptide:
EQLGIARPRV EYPAHQA
conjugated to BSA by a CDI linker, corresponding to N terminal amino acids 23-39 of Pig Secretogranin V (45-61 of pro-Pig Secretogranin V). -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.065% Sodium azide -
Concentration information loading... -
特記事項（精製）
Partially purified, caprylic acid and ammonium sulphate precipitative clean-up. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22699の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro. -
配列類似性
Belongs to the 7B2 family. -
翻訳後修飾
Proteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides.
Sulfated on tyrosine residues. -
細胞内局在
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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参照データベース
- Entrez Gene: 6447 Human
- Entrez Gene: 19064 Mouse
- Entrez Gene: 20394 Mouse
- Entrez Gene: 24677 Rat
- Entrez Gene: 25719 Rat
- Omim: 173120 Human
- SwissProt: P05408 Human
- SwissProt: P10601 Mouse
see all -
別名
- 7B2 antibody
- 7B2 protein antibody
- 7B2_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Secretogranin V antibody (ab22699)ab75197 (1µg/ml) staining secretogranin V in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of Islets of Langerhans.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-Secretogranin V antibody (ab22699)ICC/IF image of ab22699 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22699, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab22699 は 3 報の論文で使用されています。
- Zheng B et al. A three-gene panel that distinguishes benign from malignant thyroid nodules. Int J Cancer 136:1646-54 (2015). PubMed: 25175491
- Sato A et al. Distribution of 7B2 (secretogranin V)-like immunoreactivity in the Japanese red-bellied newt (Cynops pyrrhogaster) pituitary. Tissue Cell 42:176-80 (2010). IHC-Fr ; Newt . PubMed: 20434189
- McGrath MF & de Bold AJ Transcriptional analysis of the mammalian heart with special reference to its endocrine function. BMC Genomics 10:254 (2009). IHC . PubMed: 19486520
