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AB220900

Anti-Sec8 抗体

Anti-Sec8 antibody

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(1 Publication)

Rabbit Polyclonal Sec8 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EXOC4 aa 500-700.

別名を表示する

KIAA1699, SEC8, SEC8L1, EXOC4, Exocyst complex component 4, Exocyst complex component Sec8

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Sec8 antibody (AB220900)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sec8 antibody (AB220900)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A431 cells labeling Sec8 with ab220900 at 4 μg/mL (green).

Western blot - Anti-Sec8 antibody (AB220900)
  • WB

Supplier Data

Western blot - Anti-Sec8 antibody (AB220900)

All lanes:

Western blot - Anti-Sec8 antibody (ab220900) at 1/100 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Predicted band size: 110 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human EXOC4 aa 500-700. The exact immunogen used to generate this antibody is proprietary information.

Q96A65

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Sec8 also known as Exocyst complex component 4 (EXOC4) is a protein with a mass of approximately 100 kDa. It acts as a critical component of the exocyst complex which is essential for the tethering of vesicles to specific sites on the plasma membrane. Sec8 shows high expression in a variety of human tissues including the brain kidney and intestine. By anchoring vesicles it plays an important role in exocytosis and the regulation of membrane dynamics.
Biological function summary

The Sec8 protein contributes to cellular events by serving as a part of the exocyst complex a multiprotein assembly involved in vesicle trafficking. This complex consists of eight proteins including Sec3 Exo70 and Sec15. The assembly coordinates precise vesicle delivery facilitating processes such as cell growth migration and polarity establishment. Its activity is important for the proper functioning of secretory pathways ensuring communication and material transport across cell membranes.

Pathways

Sec8 operates within the secretory pathway that manages delivery and distribution of materials inside cells. It is also involved in the sustainable function of the polarized secretion pathway an essential process that ensures cellular asymmetry and directional growth. Sec8's interaction with proteins like Rab family GTPases is significant in these pathways which are responsible for guiding vesicle movement and anchoring to the target membrane.

Sec8 associations have been identified in relation to cancer and impaired cognitive function. Alterations in the expression or function of Sec8 may disrupt vesicle trafficking leading to tumorigenesis or neurodevelopmental disorders. For example within cancer this disruption can affect cell adhesion and migration promoting metastasis. Additionally interactions with proteins such as Sec6 another exocyst complex member further illustrate the interconnected impact of Sec8 dysregulation on diseases.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
See full target information EXOC4
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 51:10292-10308 PubMed37650637

2023

A genetic screen identifies BEND3 as a regulator of bivalent gene expression and global DNA methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Lounis Yakhou,Anaelle Azogui,Nikhil Gupta,Julien Richard Albert,Fumihito Miura,Laure Ferry,Kosuke Yamaguchi,Sarah Battault,Pierre Therizols,Frédéric Bonhomme,Elouan Bethuel,Arpita Sarkar,Maxim V C Greenberg,Paola B Arimondo,Gael Cristofari,Olivier Kirsh,Takashi Ito,Pierre-Antoine Defossez
View all publications
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代替製品

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