Anti-SEC62 抗体 (ab168843)
Key features and details
- Rabbit polyclonal to SEC62
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SEC62 antibody
SEC62 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SEC62 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide, corresponding to a region within amino acids 349-399 of Human SEC62 (NP_003253.1).
-
ポジティブ･コントロール
- HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab168843 was affinity purified using an epitope specific to SEC62 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168843の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 46 kDa.
|
|
| IP |
Use at 2-10 µg/mg of lysate.
|
| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 46 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
-
機能
Required for preprotein translocation. -
配列類似性
Belongs to the SEC62 family. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 744481 Chimpanzee
- Entrez Gene: 101150184 Gorilla
- Entrez Gene: 7095 Human
- Entrez Gene: 69276 Mouse
- Entrez Gene: 100174729 Orangutan
- Entrez Gene: 696989 Rhesus monkey
- Omim: 602173 Human
- SwissProt: Q99442 Human
see all -
別名
- Dtrp1 antibody
- FLJ32803 antibody
- hTP-1 antibody
see all
画像
-
Western blot - Anti-SEC62 antibody (ab168843)All lanes : Anti-SEC62 antibody (ab168843) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 46 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-SEC62 antibody (ab168843)Detection of SEC62 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168843 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab168843 は 1 報の論文で使用されています。
- OhAinle M et al. A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV. Elife 7:N/A (2018). PubMed: 30520725