Anti-SEC23IP 抗体 (ab70338)
Key features and details
- Rabbit polyclonal to SEC23IP
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SEC23IP antibody
SEC23IP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SEC23IP -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residues 1 and 50 of human SEC23IP (NP_009121.1)
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ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab70338 was affinity purified using an epitope specific to SEC23IP immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70338の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | ||
| WB | (1) |
| 特記事項 |
|---|
WB: 1/2500 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 111 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Plays a role in the organization of endoplasmic reticulum exit sites. -
組織特異性
Ubiquitously expressed with stronger levels detected in heart, liver and skeletal muscle. -
配列類似性
Belongs to the PA-PLA1 family.
Contains 1 DDHD domain.
Contains 1 SAM (sterile alpha motif) domain. -
細胞内局在
Cytoplasmic vesicle > COPII-coated vesicle membrane. Endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 11196 Human
- SwissProt: Q9Y6Y8 Human
- Unigene: 435004 Human
- Unigene: 667094 Human
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別名
- MSTP053 antibody
- p125 antibody
- P125A antibody
see all
画像
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Western blot - Anti-SEC23IP antibody (ab70338)All lanes : Anti-SEC23IP antibody (ab70338) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 111 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Anti-SEC23IP antibody (ab70338)Detection of Human SEC23IP by Western Blot of Immunprecipitate. Lane 1: HeLa whole cell lysates immunoprecipitated using ab70338 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG used for immunoprecipitation. All lanes: ab70338 at 1µg/ml. Chemiluminescence with exposure times of 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab70338 は 1 報の論文で使用されています。
- Aghazadeh Y et al. Identification of Sec23ip, Part of 14-3-3? Protein Network, as a Regulator of Acute Steroidogenesis in MA-10 Leydig Cells. Endocrinology 161:N/A (2020). PubMed: 31875919