Anti-SDS3 抗体 [EPR15000] (ab184555)
![Western blot - Anti-SDS3 antibody [EPR15000] (ab184555)](https://www.abcam.co.jp/ps/products/184/ab184555/Images/ab184555-217018-anti-sds3-antibody-epr15000-western-blot.jpg "Western blot - Anti-SDS3 antibody [EPR15000] (ab184555)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15000] to SDS3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SDS3 antibody [EPR15000]
SDS3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15000] to SDS3 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Raji, HeLa, K562 and Molt-4 cell lysates; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15000 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184555の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/250.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa).
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/250. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa). |
ターゲット情報
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機能
Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways (By similarity). May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. -
組織特異性
Expressed in various cancer cell ines. -
配列類似性
Belongs to the SDS3 family. -
ドメイン
The C-terminus is involved in transcriptional repression by HDAC-independent mechanisms. - Information by UniProt
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参照データベース
- Entrez Gene: 64426 Human
- Entrez Gene: 71954 Mouse
- Entrez Gene: 360819 Rat
- Omim: 608250 Human
- SwissProt: Q9H7L9 Human
- SwissProt: Q8BR65 Mouse
- Unigene: 416630 Human
- Unigene: 602312 Human
see all -
別名
- 2400003N08Rik antibody
- 2410008L21Rik antibody
- 45 kDa Sin3 associated polypeptide antibody
see all
画像
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Western blot - Anti-SDS3 antibody [EPR15000] (ab184555)All lanes : Anti-SDS3 antibody [EPR15000] (ab184555) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Molt-4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-SDS3 antibody [EPR15000] (ab184555)](https://www.abcam.co.jp/ps/products/184/ab184555/Images/ab184555-217017-anti-sds3-antibody-epr15000-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SDS3 antibody [EPR15000] (ab184555)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SDS3 with ab184555 at 1/250 dilution (red) compared to a Rabbit monoclonal isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibodyat 1/150 dilution.
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![Anti-SDS3 antibody [EPR15000] (ab184555)](https://www.abcam.co.jp/ps/products/184/ab184555/Images/ab184555-3-benefits-of-recombinant-antibodies.png)
Anti-SDS3 antibody [EPR15000] (ab184555)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab184555 は 2 報の論文で使用されています。
- Banks CAS et al. Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly. Sci Rep 8:13712 (2018). PubMed: 30209338
- Banks CAS et al. A Structured Workflow for Mapping Human Sin3 Histone Deacetylase Complex Interactions Using Halo-MudPIT Affinity-Purification Mass Spectrometry. Mol Cell Proteomics 17:1432-1447 (2018). PubMed: 29599190