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リコンビナントRabMAb

Anti-SDHAF1 抗体 [EPR13380] (ab185222)

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Immunocytochemistry/ Immunofluorescence - Anti-SDHAF1 antibody [EPR13380] (ab185222)
  • Western blot - Anti-SDHAF1 antibody [EPR13380] (ab185222)
  • Anti-SDHAF1 antibody [EPR13380] (ab185222)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13380] to SDHAF1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SDHAF1 antibody [EPR13380]
    SDHAF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13380] to SDHAF1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat, MCF7 and 293 cell lysates; 293 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.01% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13380

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185222の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 13 kDa).

This product detects the target protein in mitochondrial lysates (17 kDa). In our hands we are unable to detect the target protein in whole cell lysates.
ICC/IF
1/100.
特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 13 kDa).

This product detects the target protein in mitochondrial lysates (17 kDa). In our hands we are unable to detect the target protein in whole cell lysates.
ICC/IF
1/100.

ターゲット情報

  • 機能

    Plays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II.

  • 組織特異性

    Ubiquitously expressed.

  • パスウェイ

    Cofactor biosynthesis; iron-sulfur cluster biosynthesis.

  • 関連疾患

    Mitochondrial complex II deficiency

  • 配列類似性

    Belongs to the complex I LYR family. SDHAF1 subfamily.

  • 細胞内局在

    Mitochondrion matrix.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • LYR motif containing 8 antibody
    • LYR motif-containing protein 8 antibody
    • LYRM8 antibody
    • mitochondrial antibody
    • SDH assembly factor 1 antibody
    • SDHAF1 antibody
    • SDHF1_HUMAN antibody
    • Succinate dehydrogenase assembly factor 1 antibody
    • Succinate dehydrogenase assembly factor 1 mitochondrial antibody
    • Succinate dehydrogenase complex assembly factor 1 antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-SDHAF1 antibody [EPR13380] (ab185222)
    Immunocytochemistry/ Immunofluorescence - Anti-SDHAF1 antibody [EPR13380] (ab185222)

    Immunofluorescent analysis of 293 cells (4% paraformaldehyde-fixed) labeling SDHAF1 with ab185222 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and Counterstained with DAPI (blue).

  • Western blot - Anti-SDHAF1 antibody [EPR13380] (ab185222)
    Western blot - Anti-SDHAF1 antibody [EPR13380] (ab185222)
    All lanes : Anti-SDHAF1 antibody [EPR13380] (ab185222) at 1/1000 dilution

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : MCF7 mitochondria fraction lysate
    Lane 3 : MCF7 mitochondria control lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 13 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-SDHAF1 antibody [EPR13380] (ab185222)
    Anti-SDHAF1 antibody [EPR13380] (ab185222)

参考文献 (0)

ab185222 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185222 は論文での使用が確認できていません。

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