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Anti-SDF4 抗体 (ab113413)

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Western blot - Anti-SDF4 antibody (ab113413)
  • Flow Cytometry - Anti-SDF4 antibody (ab113413)
  • Immunocytochemistry/ Immunofluorescence - Anti-SDF4 antibody (ab113413)

Key features and details

  • Goat polyclonal to SDF4
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SDF4 antibody
    SDF4 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to SDF4

  • 由来種

    Goat

  • 特異性

    ab113413 is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2).

  • アプリケーション

    適用あり: Flow Cyt, ICC/IF, IHC-P, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Goat, Cow, Dog

  • 免疫原

    Synthetic peptide:

    EYKVKFLASKGHSEK

    by a Cysteine residue linker, corresponding to internal sequence amino acids 161-175 of Human SDF4 (NP_057631.1; NP_057260.2).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: U251, HeLa and Jurkat cell lysate. Flow Cyt: A431 cells ICC/IF: U2OS cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    ab11343 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab113413の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).

1 hour primary incubation is recommended for this product.
特記事項
Flow Cyt
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).

1 hour primary incubation is recommended for this product.

ターゲット情報

画像

  • Western blot - Anti-SDF4 antibody (ab113413)
    Western blot - Anti-SDF4 antibody (ab113413)
    All lanes : Anti-SDF4 antibody (ab113413) at 0.1 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : U251 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 42 kDa



    Lysates in RIPA buffer. Detected by chemiluminescence.
  • Flow Cytometry - Anti-SDF4 antibody (ab113413)
    Flow Cytometry - Anti-SDF4 antibody (ab113413)
    Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Immunocytochemistry/ Immunofluorescence - Anti-SDF4 antibody (ab113413)
    Immunocytochemistry/ Immunofluorescence - Anti-SDF4 antibody (ab113413)

    Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).

参考文献 (0)

ab113413 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab113413 は論文での使用が確認できていません。

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