Anti-SCYL1 抗体 (ab95074)
Key features and details
- Rabbit polyclonal to SCYL1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SCYL1 antibody
SCYL1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SCYL1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
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免疫原
Synthetic peptide, corresponding to a region between residues 758 and 808 of Human SCYL1 (NP_065731.3).
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ポジティブ･コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab95074 was affinity purified using an epitope specific to SCYL1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95074の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 90 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 90 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro.
Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the protein kinase superfamily.
Contains 3 HEAT repeats.
Contains 1 protein kinase domain. -
ドメイン
The protein kinase domain is predicted to be catalytically inactive. -
細胞内局在
Nucleus; Cytoplasm > cytoskeleton > centrosome. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus > cis-Golgi network. Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner; Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic during interphase and centrosomal during mitosis, it localizes to the centrosomes in a microtubule-independent manner and Cytoplasm. Cytoplasmic throughout the cell cycle. - Information by UniProt
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参照データベース
- Entrez Gene: 57410 Human
- Entrez Gene: 78891 Mouse
- Omim: 607982 Human
- SwissProt: Q96KG9 Human
- SwissProt: Q9EQC5 Mouse
- Unigene: 238839 Human
- Unigene: 276063 Mouse
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別名
- Coated vesicle associated kinase of 90 kDa antibody
- Coated vesicle-associated kinase of 90 kDa antibody
- CVAK90 antibody
see all
画像
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Western blot - Anti-SCYL1 antibody (ab95074)All lanes : Anti-SCYL1 antibody (ab95074) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 90 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-SCYL1 antibody (ab95074)Detection of SCYL1 by Western Blot of Immunprecipitate.
ab95074 at 1µg/ml staining SCYL1 in HeLa whole cell lysate immunoprecipitated using ab95074 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab95074 は 1 報の論文で使用されています。
- Amano G et al. SCYL1 arginine methylation by PRMT1 is essential for neurite outgrowth via Golgi morphogenesis. Mol Biol Cell 31:1963-1973 (2020). PubMed: 32583741