Anti-SCUBE1 抗体 (ab105358)
Key features and details
- Rabbit polyclonal to SCUBE1
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SCUBE1 antibody
SCUBE1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SCUBE1 -
由来種
Rabbit -
特異性
ab105358 is predicted to not crossreact with other SCUBE members. -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Human -
免疫原
A 14 amino acid peptide near the center of Human SCUBE1 (NP_766638).
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ポジティブ･コントロール
- WB: Daudi cell lysate ICC: Daudi cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105358の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa.
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| ICC |
Use a concentration of 20 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa. |
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ICC
Use a concentration of 20 µg/ml. |
ターゲット情報
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機能
Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology. -
組織特異性
Detected in endothelial cells. Highly expressed in platelets. Stored in platelet alpha granules, and transferrred to the cell surface upon activation and aggregation. A smaller form, probably produced by limited proteolysis, after being released from the storage granules, is associated with thrombus and localized with the subendothelial matrices in atherosclerotic plaques. -
配列類似性
Contains 1 CUB domain.
Contains 9 EGF-like domains. -
翻訳後修飾
N-glycosylated.
Could be proteolytically cleaved to release a smaller active fragment. -
細胞内局在
Secreted. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 80274 Human
- Omim: 611746 Human
- SwissProt: Q8IWY4 Human
- Unigene: 133995 Human
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別名
- SCUB1_HUMAN antibody
- SCUBE 1 antibody
- SCUBE1 antibody
see all
画像
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Western blot - Anti-SCUBE1 antibody (ab105358)Anti-SCUBE1 antibody (ab105358) at 1 µg/ml + Daudi cell lysate at 15 µg
Predicted band size: 108 kDa -
Immunocytochemistry - Anti-SCUBE1 antibody (ab105358)Immunocytochemical analysis of SCUBE1 in Daudi cells using ab105358 at 20 µg/ml. -
Immunocytochemistry - Anti-SCUBE1 antibody (ab105358)Immunofluorescence of SCUBE1 in Daudi cells using ab105358 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab105358 は 3 報の論文で使用されています。
- Zhao J et al. CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma. J Transl Med 20:520 (2022). PubMed: 36348351
- Sun W et al. SCUBE1 Controls BMPR2-Relevant Pulmonary Endothelial Function: Implications for Diagnostic Marker Development in Pulmonary Arterial Hypertension. JACC Basic Transl Sci 5:1073-1092 (2020). PubMed: 33294740
- Del Valle I et al. A genomic atlas of human adrenal and gonad development. Wellcome Open Res 2:25 (2017). IHC ; Human . PubMed: 28459107