Anti-SCP1 抗体 [EP7918] (ab175191)
![Western blot - Anti-SCP1 antibody [EP7918] (ab175191)](https://www.abcam.co.jp/ps/products/175/ab175191/Images/ab175191-515764-anti-scp1-antibody-ep7918-western-blot-human-mouse-rat-testis.jpg "Western blot - Anti-SCP1 antibody [EP7918] (ab175191)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7918] to SCP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SCP1 antibody [EP7918]
SCP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP7918] to SCP1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human SCP1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15431 -
ポジティブ･コントロール
- WB: Human, Mouse, and Rat testis lysate. IHC-P: Human, Mouse, and Rat testis tissue.
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特記事項
This product has switched from a hybridoma to recombinant production method on 31st July 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP7918 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175191の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (3) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| WB |
1/1000. Predicted molecular weight: 114 kDa.
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| 特記事項 |
|---|
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Predicted molecular weight: 114 kDa. |
ターゲット情報
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機能
Major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. -
組織特異性
Testis. -
ドメイン
Consists of an alpha-helical stretch of 700 AA residues, flanked by N- and C-terminal globular domains. The C-terminal domain has DNA-binding capacity. -
細胞内局在
Nucleus. Chromosome. In tripartite segments of synaptonemal complexes, between lateral elements in the nucleus. Found only where the chromosome cores are synapsed. Its N-terminus is found towards the center of the synaptonemal complex while the C-terminus extends well into the lateral domain of the synaptonemal complex. - Information by UniProt
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参照データベース
- Entrez Gene: 6847 Human
- Entrez Gene: 20957 Mouse
- Entrez Gene: 25276 Rat
- Omim: 602162 Human
- SwissProt: Q15431 Human
- SwissProt: Q62209 Mouse
- SwissProt: Q03410 Rat
- Unigene: 112743 Human
see all -
別名
- Cancer/testis antigen 8 antibody
- CT8 antibody
- HOM TES 14 antibody
see all
画像
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Western blot - Anti-SCP1 antibody [EP7918] (ab175191)All lanes : Anti-SCP1 antibody [EP7918] (ab175191) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Mouse testis lysate
Lane 3 : Rat testis lysate
Lysates/proteins at 15 µg per lane.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 114 kDa
Exposure time: 60 seconds -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)](https://www.abcam.co.jp/ps/products/175/ab175191/Images/ab175191-515766-anti-scp1-antibody-ep7918-immunohistochemistry-human-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling SCP1 with ab175191 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human testis. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)](https://www.abcam.co.jp/ps/products/175/ab175191/Images/ab175191-515767-anti-scp1-antibody-ep7918-immunohistochemistry-mouse-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling SCP1 with ab175191 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse testis. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)](https://www.abcam.co.jp/ps/products/175/ab175191/Images/ab175191-515768-anti-scp1-antibody-ep7918-immunohistochemistry-rat-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody [EP7918] (ab175191)Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling SCP1 with ab175191 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat testis. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab175191 は 2 報の論文で使用されています。
- Min Z et al. Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability. iScience 25:105552 (2022). PubMed: 36444304
- Li Y et al. Sohlh1 is required for synaptonemal complex formation by transcriptionally regulating meiotic genes during spermatogenesis in mice. Mol Reprod Dev 86:252-264 (2019). PubMed: 30614095