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Anti-SCNN1B (phospho T615) 抗体 (ab79172)

Submit a review Q&A (2)References (1)
Western blot - Anti-SCNN1B (phospho T615) antibody (ab79172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (ab79172)
  • Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (ab79172)

Key features and details

  • Rabbit polyclonal to SCNN1B (phospho T615)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SCNN1B (phospho T615) antibody
    SCNN1B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to SCNN1B (phospho T615)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human, African green monkey
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthesized phosphopeptide derived from human SCNN1B around the phosphorylation site of threonine 615 (P-G-TP-P-P).

  • ポジティブ･コントロール

    • Human brain tissue; extracts from HeLa cells; COS7 cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab79172の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/1000. Predicted molecular weight: 73 kDa.
IHC-P
1/50 - 1/100.
ICC/IF
1/500 - 1/1000.
特記事項
WB
1/500 - 1/1000. Predicted molecular weight: 73 kDa.
IHC-P
1/50 - 1/100.
ICC/IF
1/500 - 1/1000.

ターゲット情報

  • 機能

    Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.

  • 関連疾患

    Pseudohypoaldosteronism 1, autosomal recessive
    Liddle syndrome
    Bronchiectasis with or without elevated sweat chloride 1

  • 配列類似性

    Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily.

  • 翻訳後修飾

    Phosphorylated on serine and threonine residues.

  • 細胞内局在

    Apical cell membrane. Apical membrane of epithelial cells.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Amiloride sensitive sodium channel subunit beta antibody
    • Amiloride-sensitive sodium channel subunit beta antibody
    • Beta NaCH antibody
    • Beta-ENaC antibody
    • Beta-NaCH antibody
    • ENaC beta antibody
    • ENaCB antibody
    • Epithelial Na(+) channel subunit beta antibody
    • Epithelial Na+ channel beta subunit antibody
    • Epithelial Na+ channel subunit beta antibody
    • Epithelial sodium channel beta 2 subunit antibody
    • Epithelial sodium channel beta 3 subunit antibody
    • Nonvoltage gated sodium channel 1 beta subunit antibody
    • Nonvoltage gated sodium channel 1 subunit beta antibody
    • Nonvoltage-gated sodium channel 1 subunit beta antibody
    • SCNEB antibody
    • SCNN 1B antibody
    • SCNN1B antibody
    • SCNNB_HUMAN antibody
    • Sodium channel nonvoltage gated 1 beta (Liddle syndrome) antibody
    • Sodium channel nonvoltage gated 1 beta antibody
    see all

画像

  • Western blot - Anti-SCNN1B (phospho T615) antibody (ab79172)
    Western blot - Anti-SCNN1B (phospho T615) antibody (ab79172)
    All lanes : Anti-SCNN1B (phospho T615) antibody (ab79172) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HeLa cells with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 73 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (ab79172)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (ab79172)
    Immunohistochemiscal analysis of SCNN1B in paraffin embedded human brain tissue using a 1/50 dilution of ab79172 in the presence (right) or absence (left) of the immunizing phosphopeptide.
  • Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (ab79172)
    Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (ab79172)
    Immunofluorescence analysis of SCNN1B in COS7 cells, using a 1/500 dilution of ab79172, in the presence (right) or absence (left) of the immunizing phosphopeptide.

参考文献 (1)

ab79172 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab79172 は 1 報の論文で使用されています。

  • Shimko MJ  et al. Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells. Physiol Rep 2:N/A (2014). WB ; Human . PubMed: 25347857

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

Is the immunizing peptide for ab79172 available?

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Answer

I have contacted the lab and they have told me that the immunogen sequence for ab79172 is the sequence between *******of the human sequence. If there is anything else I can help you with, please let me know.

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Question

Is the immunizing peptide for ab79172 available?

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Answer

Thank you for contacting Abcam. I have heard back from the lab and they have told me that we currently do not have enough of the immunizing peptide used to generate ab79172 to sell. I am sorry that I could not provide you with the immunizing peptide, but it if there is anything else I can help you with, please let me know.

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