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AB309474

Anti-SCN3A 抗体 [EPR25135-66] - BSA and Azide free

Anti-SCN3A antibody [EPR25135-66] - BSA and Azide free

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Rabbit Recombinant Monoclonal SCN3A antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Rat samples.

別名を表示する

KIAA1356, NAC3, SCN3A, Sodium channel protein type 3 subunit alpha, Sodium channel protein brain III subunit alpha, Sodium channel protein type III subunit alpha, Voltage-gated sodium channel subtype III, Voltage-gated sodium channel subunit alpha Nav1.3

2 Images
Western blot - Anti-SCN3A antibody [EPR25135-66] - BSA and Azide free (AB309474)
  • WB

Supplier Data

Western blot - Anti-SCN3A antibody [EPR25135-66] - BSA and Azide free (AB309474)

This data was developed using ab309473, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human SCN2A, SCN7A, SCN8A, SCN9A, SCN10A, SCN11A, SCN1A, SCN4A, SCN5A. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 3 seconds

All lanes:

Western blot - Anti-SCN3A antibody [EPR25135-66] (<a href='/products/primary-antibodies/scn3a-antibody-epr25135-66-ab309473'>ab309473</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his tag whole cell lysate at 20 µg

Lanes 2, 9 and 10:

293T cells transfected with a Human SCN3A expression vector containing a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Human SCN2A expression vector containing a His-tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a Human SCN7A expression vector containing a His-tag whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a Human SCN8A expression vector containing a His-tag whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a Human SCN9A expression vector containing a His-tag whole cell lysate at 20 µg

Lane 7:

293T cells transfected with a Human SCN10A expression vector containing a His-tag whole cell lysate at 20 µg

Lane 8:

293T cells transfected with a Human SCN11A expression vector containing a His-tag whole cell lysate at 20 µg

Lane 11:

293T cells transfected with a Human SCN4A fragment expression vector containing a His-tag whole cell lysate at 20 µg

Lane 12:

293T cells transfected with a Human SCN5A fragment expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 320 kDa,260 kDa

false

Exposure time: 3s

Western blot - Anti-SCN3A antibody [EPR25135-66] - BSA and Azide free (AB309474)
  • WB

Supplier Data

Western blot - Anti-SCN3A antibody [EPR25135-66] - BSA and Azide free (AB309474)

This data was developed using ab309473, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : skeletal muscle tissue (PMID : 22081212), liver (PMID : 10842222). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 16912065)(PMID : 35055012). SCN3A is abundantly expressed in neuronal tissues during embryonic and neonatal stages of development and is rare in adult tissues. (PMID : 29466837, 2555170). In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-SCN3A antibody [EPR25135-66] (<a href='/products/primary-antibodies/scn3a-antibody-epr25135-66-ab309473'>ab309473</a>) at 1/1000 dilution

Lane 1:

Rat E15 brain tissue lysate at 50 µg

Lane 2:

Rat skeletal muscle tissue lysate at 50 µg

Lane 3:

Rat liver tissue lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 260 kDa,320 kDa

false

Exposure time: 180s

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR25135-66

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human, Rat

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SCN3A also known as sodium channel voltage-gated type III alpha subunit or NaV1.3 plays a mechanical role in the conduction of electrical signals in excitable cells. It forms part of the voltage-gated sodium channels that are critical in the generation and propagation of action potentials. SCN3A has a molecular mass of approximately 260 kDa and is expressed broadly in the central nervous system particularly in neurons.
Biological function summary

The voltage-gated sodium channel SCN3A contributes to rapid depolarization in neuronal action potentials. It is an important component of a larger sodium channel complex which includes beta subunits that regulate the kinetic properties and localization of these channels. This enables proper neuronal signaling and communication affecting processes such as neurotransmission and response to stimuli.

Pathways

SCN3A influences the regulation of neuronal excitability and synaptic activity. It operates within the action potential pathway and has connections to the calcium signaling pathway which impacts numerous cellular processes. SCN3A functions alongside proteins such as SCN1A and SCN2A within these pathways contributing to the integrated organization of electrical signaling in neurons.

Mutations or dysregulations in SCN3A relate to epilepsy and neurodevelopmental disorders. SCN3A's interaction with SCN1A is significant as alterations in sodium channel function can lead to imbalance in neuronal excitability and seizures. These relationships highlight the importance of SCN3A in maintaining normal neural function and its potential as a therapeutic target in related neurologic conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Pore-forming subunit of Nav1.3, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues (PubMed : 24157691, PubMed : 28235671, PubMed : 29466837, PubMed : 35277491). In some secretory cell types, it also participates in cell excitability through membrane depolarization and regulates cells responsiveness to stimuli triggering secretion. For instance, it controls the release of serotonin/5-hydroxytryptamine by enterochromaffin cells and is required for both glucagon- and glucose-induced insulin secretion in pancreatic endocrine cells (By similarity).
See full target information SCN3A
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組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB85015

Anti-Nav1.7 antibody [N68/6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.7 antibody [N68/6] (AB85015)

  • 4
  • 1
Primary Antibodies

AB53724

Anti-pan Sodium Channel antibody

Western blot - Anti-pan Sodium Channel antibody (AB53724)

  • 0
  • 0
Primary Antibodies

AB248042

Anti-SPTBN1 antibody [EPR5869] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody [EPR5869] - BSA and Azide free (AB248042)

  • 0
  • 0

Abcam product promise

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