Anti-SCML2 抗体 [SCMAD14A] (ab51506)
![Western blot - Anti-SCML2 antibody [SCMAD14A] (ab51506)](https://www.abcam.co.jp/ps/products/51/ab51506/Images/ab51506-63460-anti-scml2-antibody-scmad14a-western-blot.jpg "Western blot - Anti-SCML2 antibody [SCMAD14A] (ab51506)")
Key features and details
- Mouse monoclonal [SCMAD14A] to SCML2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-SCML2 antibody [SCMAD14A] -
製品の詳細
Mouse monoclonal [SCMAD14A] to SCML2 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment, C terminal. (Human)
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ポジティブ･コントロール
- HeLa Whole cell lysate, NIH3T3 whole cell lysate, and F2408 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
ab51506 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
ポリ/モノ
モノクローナル -
クローン名
SCMAD14A -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51506の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 77.3 kDa).
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| 特記事項 |
|---|
|
WB
1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 77.3 kDa). |
ターゲット情報
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機能
Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. -
組織特異性
Highly expressed in placenta, thymus and testis. Detected at lower levels in brain, liver, skeletal muscle, pancreas and ovary. -
配列類似性
Belongs to the SCM family.
Contains 2 MBT repeats.
Contains 1 SAM (sterile alpha motif) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10389 Human
- Entrez Gene: 107815 Mouse
- Entrez Gene: 367793 Rat
- Omim: 300208 Human
- SwissProt: Q9UQR0 Human
- Unigene: 495774 Human
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別名
- SCML2 antibody
- SCML2_HUMAN antibody
- sex comb on midleg like 2 (Drosophila) antibody
see all
画像
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Western blot - Anti-SCML2 antibody [SCMAD14A] (ab51506)Anti-SCML2 antibody [SCMAD14A] (ab51506) at 1/200 dilution + HeLa whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 77.3 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Same results obtained using NIH3T3 whole cell lysate, and F2408 whole cell lysate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab51506 は 1 報の論文で使用されています。
- Galoian K et al. Epigenetic regulation of embryonic stem cell marker miR302C in human chondrosarcoma as determinant of antiproliferative activity of proline-rich polypeptide 1. Int J Oncol 47:465-72 (2015). PubMed: 26094604