Anti-SCGB3A2 抗体 [EPR11463] (ab181853)
![Immunocytochemistry/ Immunofluorescence - Anti-SCGB3A2 antibody [EPR11463] (ab181853)](https://www.abcam.co.jp/ps/products/181/ab181853/Images/ab181853-262048-anti-scgb3a2-antibody-epr11463-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SCGB3A2 antibody [EPR11463] (ab181853)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11463] to SCGB3A2
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SCGB3A2 antibody [EPR11463]
SCGB3A2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11463] to SCGB3A2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Calu 3 cell lysate; Human SCGB3A2 recombinant protein; A549 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11463 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181853の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
|
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| ICC/IF |
1/500.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). |
|
ICC/IF
1/500. |
ターゲット情報
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組織特異性
Highly expressed in lung. -
配列類似性
Belongs to the secretoglobin family. UGRP subfamily. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 117156 Human
- Omim: 606531 Human
- SwissProt: Q96PL1 Human
- Unigene: 483765 Human
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別名
- LU103 antibody
- Pneumo secretory protein 1 antibody
- PnSP-1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-SCGB3A2 antibody [EPR11463] (ab181853)
Immunocytochemistry/Immunofluorescence analysis of A549 (human lung carcinoma) labelling SCGB3A2 with purified ab181853 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Immunocytochemistry/ Immunofluorescence - Anti-SCGB3A2 antibody [EPR11463] (ab181853)](https://www.abcam.co.jp/ps/products/181/ab181853/Images/ab181853-212826-anti-scgb3a2-antibody-epr11463-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SCGB3A2 antibody [EPR11463] (ab181853)Immunofluorescent analysis of A549 cells (paraformaldehyde-fixed, 4%) labeling SCGB3A2 with ab181853 at 1/500 dilution followed by Goat anti rabbit IgG Alexa Fluor 555) secondary at 1/200 dilution and counter-stained with DAPI.
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![Western blot - Anti-SCGB3A2 antibody [EPR11463] (ab181853)](https://www.abcam.co.jp/ps/products/181/ab181853/Images/ab181853-212825-anti-scgb3a2-antibody-epr11463-western-blot.jpg)
Western blot - Anti-SCGB3A2 antibody [EPR11463] (ab181853)Anti-SCGB3A2 antibody [EPR11463] (ab181853) at 1/1000 dilution + Human SCGB3A2 recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution
Predicted band size: 10 kDa -
![Western blot - Anti-SCGB3A2 antibody [EPR11463] (ab181853)](https://www.abcam.co.jp/ps/products/181/ab181853/Images/ab181853-212822-anti-scgb3a2-antibody-epr11463-western-blot.jpg)
Western blot - Anti-SCGB3A2 antibody [EPR11463] (ab181853)Anti-SCGB3A2 antibody [EPR11463] (ab181853) at 1/1000 dilution + Calu 3 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution
Predicted band size: 10 kDa -
![Anti-SCGB3A2 antibody [EPR11463] (ab181853)](https://www.abcam.co.jp/ps/products/181/ab181853/Images/ab181853-5-benefits-of-recombinant-antibodies.png)
Anti-SCGB3A2 antibody [EPR11463] (ab181853)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (6)
ab181853 は 6 報の論文で使用されています。
- Han L et al. Uterus globulin associated protein 1 (UGRP1) binds podoplanin (PDPN) to promote a novel inflammation pathway during Streptococcus pneumoniae infection. Clin Transl Med 12:e850 (2022). PubMed: 35652821
- He P et al. A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates. Cell 185:4841-4860.e25 (2022). PubMed: 36493756
- He D et al. Single-cell RNA sequencing reveals heterogeneous tumor and immune cell populations in early-stage lung adenocarcinomas harboring EGFR mutations. Oncogene 40:355-368 (2021). PubMed: 33144684
- Leibel SL et al. Generation of Complete Multi-Cell Type Lung Organoids From Human Embryonic and Patient-Specific Induced Pluripotent Stem Cells for Infectious Disease Modeling and Therapeutics Validation. Curr Protoc Stem Cell Biol 54:e118 (2020). PubMed: 32640120
- Jacob A et al. Differentiation of Human Pluripotent Stem Cells into Functional Lung Alveolar Epithelial Cells. Cell Stem Cell 21:472-488.e10 (2017). PubMed: 28965766
- McCauley KB et al. Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling. Cell Stem Cell 20:844-857.e6 (2017). PubMed: 28366587