Anti-SCG10 抗体 (ab21190)
Key features and details
- Goat polyclonal to SCG10
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SCG10 antibody
SCG10 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SCG10 -
由来種
Goat -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human SCG10 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q93045 -
ポジティブ・コントロール
- Human Brain lysate, A431 lysate ICC/IF: HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21190の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min). |
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WB |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 20.8 kDa.
A 1 hour primary incubation is recommended for this product. |
特記事項 |
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ICC/IF
1/100. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min). |
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 20.8 kDa. A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Is a key regulator of neurite extension through regulation of microtubule instabilily. -
組織特異性
Neuron specific. -
配列類似性
Belongs to the stathmin family. -
翻訳後修飾
Sumoylated. -
細胞内局在
Cytoplasm. Cytoplasm > perinuclear region. Cell projection > growth cone. Membrane. Cell projection > axon. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. SCG10 exists in both soluble and membrane-bound forms. - Information by UniProt
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参照データベース
- Entrez Gene: 11075 Human
- Omim: 600621 Human
- SwissProt: Q93045 Human
- Unigene: 521651 Human
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別名
- Neuron specific growth associated protein antibody
- Neuronal growth associated protein antibody
- Neuronal growth associated protein (silencer element) antibody
see all
画像
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Ab211990 staining TAF9 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211990 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Anti-SCG10 antibody (ab21190) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 20.8 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab21190 は 2 報の論文で使用されています。
- Davis EJ et al. Intrastriatal dopamine D1 antagonism dampens neural plasticity in response to motor cortex lesion. Neuroscience 146:784-91 (2007). PubMed: 17331653
- Ohkawa N et al. The microtubule destabilizer stathmin mediates the development of dendritic arbors in neuronal cells. J Cell Sci 120:1447-56 (2007). PubMed: 17389683