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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Scavenging Receptor SR-BI 抗体 [EP1556Y] - Low endotoxin, Azide free (ab176238)

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Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
  • Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI - Low endotoxin, Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647

製品の概要

  • 製品名

    Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free
    Scavenging Receptor SR-BI 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI - Low endotoxin, Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    ab176238 is the carrier-free version of ab52629.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab176238の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 80 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 80 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

  • 機能

    Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.

  • 組織特異性

    Widely expressed.

  • 配列類似性

    Belongs to the CD36 family.

  • 翻訳後修飾

    N-glycosylated.

  • 細胞内局在

    Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CD36 and LIMPII analogous 1 antibody
    • CD36 antibody
    • CD36 Antigen like 1 antibody
    • CD36 antigen-like 1 antibody
    • CD36L1 antibody
    • CLA 1 antibody
    • CLA-1 antibody
    • CLA1 antibody
    • Collagen type I receptor antibody
    • HDLQTL6 antibody
    • MGC138242 antibody
    • SCARB1 antibody
    • Scavebger Receptor Class B Member 1 antibody
    • Scavenger receptor class B member 1 antibody
    • Scavenger Receptor Class B Type 1 antibody
    • SCRB1_HUMAN antibody
    • SR BI antibody
    • SR-BI antibody
    • SRB1 antibody
    • SRBI antibody
    • Thrombospondin receptor like 1 antibody
    • thrombospondin receptor-like 1 antibody
    see all

画像

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
    All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : SCARB1 knockout HEK-293T cell lysate
    Lane 3 : Human Liver cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Observed band size: 70,75 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-Scavenging Receptor SR-BI antibody [EP1556Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab52629 was shown to bind specifically to Scavenging Receptor SR-BI. A band was observed at 70/75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SCARB1 knockout cell line ab282646 (knockout cell lysate ab283046). To generate this image, wild-type and SCARB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
    Immunohistochemical staining of paraffin embedded human liver with purified ab52629 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52629).

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
    Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: Human liver whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab52629 observed at 80 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab52629 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. Ab52629 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176238).

  • Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
    Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)

参考文献 (0)

ab176238 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab176238 は論文での使用が確認できていません。

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