Anti-SCAMP2/SC2 抗体 [EPR10185] (ab154181)
![Western blot - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)](https://www.abcam.co.jp/ps/products/154/ab154181/Images/ab154181-160123-anti-scamp2-antibody-epr10185-western-blot.jpg "Western blot - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10185] to SCAMP2/SC2
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SCAMP2/SC2 antibody [EPR10185]
SCAMP2/SC2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10185] to SCAMP2/SC2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human SCAMP2/SC2. The exact sequence is proprietary.
Database link: O15127 -
ポジティブ･コントロール
- HepG2, 293T and Human tonsil lysates, Human pancreas tissue, permeabilized 293T cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10185 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154181の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
|
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the SCAMP family. -
細胞内局在
Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10066 Human
- GenBank: NP_005688 Human
- Omim: 606912 Human
- SwissProt: O15127 Human
- Unigene: 458917 Human
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別名
- SCAM2_HUMAN antibody
- SCAMP2 antibody
- Secretory carrier membrane protein 2 antibody
- Secretory carrier-associated membrane protein 2 antibody
画像
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Western blot - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)All lanes : Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181) at 1/1000 dilution
Lane 1 : HepG2 lysates
Lane 2 : 293T lysates
Lane 3 : Human tonsil lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)](https://www.abcam.co.jp/ps/products/154/ab154181/Images/ab154181-160122-anti-scamp2-antibody-epr10185-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SCAMP2/SC2 with ab154181 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)](https://www.abcam.co.jp/ps/products/154/ab154181/Images/ab154181-160121-anti-scamp2-antibody-epr10185-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)Intracellular flow cytometric analysis of permeabilized 293T cells with ab154181 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)](https://www.abcam.co.jp/ps/products/154/ab154181/Images/ab154181-160120-anti-scamp2-antibody-epr10185-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)Immunohistochemical analysis of paraffin embedded Human prostate hyperplasia tissue using ab154181 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)](https://www.abcam.co.jp/ps/products/154/ab154181/Images/ab154181-160119-anti-scamp2-antibody-epr10185-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154181 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)](https://www.abcam.co.jp/ps/products/154/ab154181/Images/ab154181-160118-anti-scamp2-antibody-epr10185-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue using ab154181 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)](https://www.abcam.co.jp/ps/products/154/ab154181/Images/ab154181-160117-anti-scamp2-antibody-epr10185-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154181 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)](https://www.abcam.co.jp/ps/products/154/ab154181/Images/ab154181-160116-anti-scamp2-antibody-epr10185-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154181 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)](https://www.abcam.co.jp/ps/products/154/ab154181/Images/ab154181-9-benefits-of-recombinant-antibodies.png)
Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154181 は論文での使用が確認できていません。