Anti-SCAMP1 抗体 (ab3430)
Key features and details
- Rabbit polyclonal to SCAMP1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SCAMP1 antibody -
製品の詳細
Rabbit polyclonal to SCAMP1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Pig
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免疫原
Synthetic peptide corresponding to Human SCAMP1 aa 1-17.
Sequence:MSDFDSNPFADPDLNNP
(Peptide available asab4969) -
ポジティブ･コントロール
- WB: U-87MG, Neuro-2a, SH-SY5Y, SK-N-AS, T98G, IMR-32, Rat brain tissue and Wild-type HeLa cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3430の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa).
By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate. |
| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa). By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate. |
ターゲット情報
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機能
Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface. -
組織特異性
Widely expressed, with highest expression in brain. -
配列類似性
Belongs to the SCAMP family. -
細胞内局在
Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9522 Human
- Entrez Gene: 107767 Mouse
- Entrez Gene: 397477 Pig
- Entrez Gene: 29521 Rat
- Omim: 606911 Human
- SwissProt: O15126 Human
- SwissProt: Q8K021 Mouse
- SwissProt: O77735 Pig
see all -
別名
- SCAM1_HUMAN antibody
- SCAMP 1 antibody
- SCAMP 37 antibody
see all
画像
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Western blot - Anti-SCAMP1 antibody (ab3430)All lanes : Anti-SCAMP1 antibody (ab3430) at 1 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SCAMP1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 38 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab3430 observed at 36 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab3430 was shown to react with SCAMP1 in wild-type HeLa cells in western blot. The bands observed in SCAMP1 knockout cell line ab265567 (SCAMP1 knockout cell lysate ab258184) below 36 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and SCAMP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3430 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at 1 µg/ml and a 1/20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.
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Western blot - Anti-SCAMP1 antibody (ab3430)All lanes : Anti-SCAMP1 antibody (ab3430) at 2 µg/ml
Lane 1 : U-87MG (Human primary glioblastoma cell line) lysate
Lane 2 : Neuro-2a (Mouse brain) lysate
Lane 3 : SH-SY5Y (Human derived cell line) lysate
Lane 4 : SK-N-AS (Human neuroblastoma cell line) lysate
Lane 5 : T98G (Glioblastoma cell line) lysate
Lane 6 : IMR-32 (Human neuroblastoma cell line) lysate
Lane 7 : Rat brain tissue
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/4000 dilution
Predicted band size: 38 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?Blocking buffer: 5% Milk.
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Western blot - Anti-SCAMP1 antibody (ab3430)Western blot detection of SCAMP1 on rat brain extract using ab3430.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab3430 は 2 報の論文で使用されています。
- Gerlini R et al. Double knock-out of Hmga1 and Hipk2 genes causes perinatal death associated to respiratory distress and thyroid abnormalities in mice. Cell Death Dis 10:747 (2019). PubMed: 31582725
- Jain P et al. Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein. J Biol Chem 282:34581-93 (2007). WB, Dot blot ; Hamster . PubMed: 17897946
