Anti-SC35 抗体 [SC-35] - Nuclear Speckle Marker

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

ab11826 staining SC35 - Nuclear Speckled Marker in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

Immunocytochemistry/ Immunofluorescence analysis of HEK-293T and RKO cells transiently transfected with CDX2/AS-His and co-stained for CDX2/AS-His and SC35 (ab11826). All proteins localized to the nucleus and merged images revealed co-localization of CDX2/AS with SC35.

Image from Witek, Matthew E. et al. PLoS ONE 9.8 (2014): e104293. doi: 10.1371/journal.pone.0104293. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ICC/IF

AbReview8138****

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

ab11826 (1/1000) staining SC35 (phospho) in human retinal pigment epithelial (RPE) cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.

This image is courtesy of an Abreview submitted by Dr Kirk McManus

• ICC/IF

AbReview9179****

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

ab11826 staining cultured human colon adenocarcinoma HT-29 cells.

Cells were PFA fixed and permeabilized in Triton X-100 and saponin prior to blocking with 1% BSA for 1 hour at RT. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor^® 594 conjugated goat anti-mouse IgG3 antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Eva Bartova

• ICC/IF

AbReview55219****

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

ab11826 staining SC35 in human fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% Normal Goat Serum/0.3% Triton X-100 in PBS for 60 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 1% BSA/ 0.3% Triton X-100 in PBS) for 16 hours at 4°C. An Alexa Flour^® 488 goat anti-mouse IgG (H+L), F(ab')2 Fragment Ig was used as the secondary antibody at a dilution of 1/1000.

This image is courtesy of an anonymous Abreview.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were fixed 24–48 hours after transfection using 4% paraformaldehyde, permeabilized with 0.2% triton X-100/PBS and probed with ab11826 followed by FITC conjugated secondary antibodies (green). After washing with PBS, slides were mounted using Citifluor and analysed by confocal microscopy. Cells were visualized under a Leica laser scanning confocal microscope equipped with a DM-RXE microscope and an argon-krypton laser.

Image from de Chiara C et al, PLoS One. 2009 Dec 23;4(12):e8372, Fig 3.

• IHC-P

AbReview66a246e99358c97112dec1ca****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Ly6g with ab11826 at 1/100 dilution with PBST.

Positive staining on human brain.

The section was blocked using 10% milk as blocking agent for 1 hour at 24 °C before incubation with ab11826 for 1 hour at 24°C followed by a Goat anti-mouse IgG (H+L) biotinylated antibody (undiluted).

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed.

This image is courtesy of an anonymous Abreview

• ICC/IF

AbReview48302****

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

Immunocytochemistry/ Immunofluorescence analysis of human hippocampus cells labeling SC35 with ab11826 at 1/200 dilution. Cells were fixed with formaldehyde and blocked with PBS for 1 hour at 4°C. Staining with ab11826 was carried out in PBS buffer for 12 hours at 4°C. A goat anti-mouse Alexa Fluor^® 594 secondary antibody was used at 1/1000 dilution.

This image is courtesy of an abreview submitted by Dr Sam Nowitzki, Barrow Neurological Institute.

• ICC/IF

AbReview48348****

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

Immunocytochemistry/ Immunofluorescence analysis of HEK-293 human kidney cells labeling SC35 with ab11826 at 1/400 dilution. Cells were fixed with methanol and blocked with PBS for 1 hour at 4°C. Staining with ab11826 was carried out in PBS buffer for 2 hours at 4°C. An undiluted goat anti-mouse Alexa Fluor^® 594 secondary antibody was used.

This image is courtesy of an abreview submitted by Dr Sam Nowitzki, Barrow Neurological Institute.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

Immunocytochemistry/ Immunofluorescence analysis of untransfected U-2 OS cells (A) and cells transfected with HSV US1 or US1.5 fixed and stained for FLAG (red) and SC35 (green) to identify viral proteins and nuclear speckles respectively. Transfected cells were fixed 40 h post transfection with 3.7% formaldehyde in PBS (20 min), permeabilized with 0.5% Triton X-100 in PBS (10 min), and blocked with 4% BSA in PBS (20 min) prior to incubation with Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826) and secondary antibodies in 4% BSA in PBS. DAPI was used for visualization of nuclear DNA. Scale bar  =  10 μm.

Image from Salsman, Jayme et al. PLoS Pathogens 4.7 (2008): e1000100. doi: 10.1371/journal.ppat.1000100. Fig S4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

ab11826 staining SC35 - Nuclear Speckle Marker in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

• ICC/IF

AbReview10337****

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

Immunocytochemistry/ Immunofluorescence analysis of human adenocarcinoma HT-29 (Human colorectal adenocarcinoma cell line) cells labeling SC35 with ab11826 at 1/200 dilution. Cells were fixed in paraformaldehyde and permeabilized with Triton X-100 and Saponin. Blocking of the cells was done with 1% BSA for 1 hour at 37°C; staining with ab11826 at 1/200 was carried out for 16 hours at 4°C in PBS buffer. An anti-mouse IgG3 (Alexa Fluor^® 594) secondary antibody was used at 1/200 dilution.

This image is courtesy of an Abreview submitted by Dr Eva Bartova

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

ab11826 staining SC35 - Nuclear Speckle Marker in Rin-5F cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).