Anti-SBDS 抗体 (ab154222)
Key features and details
- Rabbit polyclonal to SBDS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SBDS antibody
SBDS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SBDS -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
-
免疫原
Recombinant fragment corresponding to Human SBDS aa 1-250.
Database link: Q9Y3A5 -
ポジティブ･コントロール
- Recombinant Human SBDS protein (ab99957) can be used as a positive control in WB. 293T, A431, H1299, HepG2, Molt-4, Raji and Mouse liver lysate, HeLa cells, H1299 xenograft tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154222の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
|
|
| IHC-P |
1/100 - 1/1000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
|
IHC-P
1/100 - 1/1000. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
-
機能
Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFTUD1, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation. -
組織特異性
Widely expressed. -
関連疾患
Defects in SBDS are the cause of Shwachman-Diamond syndrome (SDS) [MIM:260400]. SDS is an autosomal recessive disorder characterized by pancreatic exocrine insufficiency, hematologic dysfunction, and skeletal abnormalities. -
配列類似性
Belongs to the SDO1/SBDS family. -
細胞内局在
Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Primarily detected in the cytoplasm, and at low levels in nucleus and nucleolus (PubMed:19602484 and PubMed:17475909). Detected in the nucleolus during G1 and G2 phase of the cell cycle, and diffusely distributed in the nucleus during S phase. Detected at the mitotic spindle. Colocalizes with the microtubule organizing center during interphase. - Information by UniProt
-
参照データベース
- Entrez Gene: 417477 Chicken
- Entrez Gene: 513237 Cow
- Entrez Gene: 51119 Human
- Entrez Gene: 66711 Mouse
- Entrez Gene: 288615 Rat
- Entrez Gene: 100049749 Xenopus laevis
- Entrez Gene: 448365 Xenopus tropicalis
- Omim: 607444 Human
see all -
別名
- 4733401P19Rik antibody
- AI836084 antibody
- CGI 97 antibody
see all
画像
-
Western blot - Anti-SBDS antibody (ab154222)All lanes : Anti-SBDS antibody (ab154222) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-SBDS antibody (ab154222)Anti-SBDS antibody (ab154222) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SBDS antibody (ab154222)Immunofluorescence analysis of methanol-fixed HeLa cells, labelling SBDS with ab154222 at 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (ab154222)Immunohistochemical analysis of paraffin embedded Human H1299 xenograft tissue labelling SBDS with ab154222 at a 1/500 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab154222 は論文での使用が確認できていません。