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AB154222

Anti-SBDS 抗体

Anti-SBDS antibody

  • JP Deleterious:医薬用外劇物

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Rabbit Polyclonal SBDS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SBDS aa 1 to C-terminus.

別名を表示する

CGI-97, SBDS, Ribosome maturation protein SBDS, Shwachman-Bodian-Diamond syndrome protein

6 Images
Western blot - Anti-SBDS antibody (AB154222)
  • WB

Unknown

Western blot - Anti-SBDS antibody (AB154222)

12% SDS PAGE

All lanes:

Western blot - Anti-SBDS antibody (ab154222) at 1/1000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Lane 3:

HepG2 whole cell lysate at 30 µg

Predicted band size: 29 kDa

false

Western blot - Anti-SBDS antibody (AB154222)
  • WB

Unknown

Western blot - Anti-SBDS antibody (AB154222)

12% SDS PAGE

All lanes:

Western blot - Anti-SBDS antibody (ab154222) at 1/1000 dilution

All lanes:

Mouse liver whole cell lysate at 50 µg

Predicted band size: 29 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-SBDS antibody (AB154222)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SBDS antibody (AB154222)

Immunofluorescence analysis of methanol-fixed HeLa cells, labelling SBDS with ab154222 at 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (AB154222)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (AB154222)

Immunohistochemical analysis of paraffin embedded Human H1299 xenograft tissue labelling SBDS with ab154222 at a 1/500 dilution.

Western blot - Anti-SBDS antibody (AB154222)
  • WB

Supplier Data

Western blot - Anti-SBDS antibody (AB154222)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-SBDS antibody (ab154222) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 29 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (AB154222)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBDS antibody (AB154222)

Immunohistochemical analysis of paraffin embedded mouse duodenum tissue stained for SBDS protein at cytosol with ab154222 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human SBDS aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3A5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SBDS also known as Shwachman-Bodian-Diamond syndrome protein participates in ribosome biogenesis. It has a molecular weight of approximately 29 kDa. SBDS is expressed in a variety of tissues with notable expression in the bone marrow and pancreas areas that require active ribosome synthesis. The protein works to assist in the assembly and maturation of the 60S ribosomal subunit associating closely with other ribosomal proteins and factors needed for proper cellular function.
Biological function summary

SBDS plays an essential role in the maturation of ribosomes and is part of the ribosomal large subunit assembly complex. It interacts with proteins such as EFL1 and Nucleophosmin highlighting its involvement in vital cellular processes. The protein contributes to protein synthesis machinery ensuring that ribosomal subunits are correctly formed and functional. SBDS is important for maintaining cellular homeostasis through its engagement in ribosome assembly.

Pathways

SBDS is involved in ribosome biogenesis a fundamental pathway for cell growth and division. This process is closely connected with proteins like eIF6 which cooperates with SBDS during the final stages of ribosomal subunit maturation. Additionally SBDS aligns with pathways related to cellular stress responses given its role in ensuring the integrity and function of ribosomes under conditions of cellular stress. These interactions demonstrate SBDS's importance in overarching cellular pathways that sustain life.

Mutations in the SBDS gene cause Shwachman-Diamond syndrome a rare congenital disorder that affects bone marrow function and pancreatic enzymes. The syndrome often results in neutropenia and pancreatic insufficiency manifesting through the disruption of ribosome biogenesis. Moreover research suggests SBDS might interact with proteins involved in other ribosomopathies connecting it indirectly to a spectrum of related disorders. Understanding SBDS’s role in these contexts provides insight into therapeutic targets and interventions for diseases involving ribosomal dysfunction.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFL1, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation.
See full target information SBDS
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。
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