Anti-SATB2 抗体 (ab69995)
Key features and details
- Rabbit polyclonal to SATB2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SATB2 antibody
SATB2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SATB2 -
由来種
Rabbit -
特異性
ab69995 does not cross-react with SATB1. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic 16 amino acid peptide near the amino terminus of the human SATB2.
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ポジティブ･コントロール
- Mouse brain tissue lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69995の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa). |
ターゲット情報
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機能
Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. -
組織特異性
High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus. -
関連疾患
Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22). -
配列類似性
Belongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain. -
翻訳後修飾
Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity. -
細胞内局在
Nucleus matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 212712 Mouse
- SwissProt: Q8VI24 Mouse
- Unigene: 145599 Mouse
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別名
- DNA binding protein SATB2 antibody
- DNA-binding protein SATB2 antibody
- FLJ21474 antibody
see all
画像
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Western blot - Anti-SATB2 antibody (ab69995)Lane 1 : Anti-SATB2 antibody (ab69995) at 2 µg/ml
Lane 2 : Anti-SATB2 antibody (ab69995) at 4 µg/ml
All lanes : Mouse brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 82 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab69995 は 6 報の論文で使用されています。
- Sun XY et al. Generation of vascularized brain organoids to study neurovascular interactions. Elife 11:N/A (2022). PubMed: 35506651
- Ou MY et al. The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development. Cell Rep 35:109290 (2021). PubMed: 34192541
- Filatova A et al. Mutations in SMARCB1 and in other Coffin-Siris syndrome genes lead to various brain midline defects. Nat Commun 10:2966 (2019). PubMed: 31273213
- He L et al. Expression and localization of special AT-rich sequence binding protein 2 in murine molar development and the pulp-dentin complex of human healthy teeth and teeth with pulpitis. Exp Ther Med 14:3507-3512 (2017). PubMed: 29042940
- Schütz B et al. Satb2-Independent Acquisition of the Cholinergic Sudomotor Phenotype in Rodents. Cell Mol Neurobiol N/A:N/A (2014). Rat . PubMed: 25239161
- Zhang J et al. Roles of SATB2 in osteogenic differentiation and bone regeneration. Tissue Eng Part A 17:1767-76 (2011). IHC-P ; Mouse . PubMed: 21385070