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Anti-SARS E2 抗体 [4A6C9] - Coronavirus (ab31951)

Submit a review Q&A (1)
Western blot - Anti-SARS E2 antibody [4A6C9] - Coronavirus (ab31951)

    Key features and details

    • Mouse monoclonal [4A6C9] to SARS E2 - Coronavirus
    • Suitable for: ELISA, WB
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-SARS E2 antibody [4A6C9] - Coronavirus

    • 製品の詳細

      Mouse monoclonal [4A6C9] to SARS E2 - Coronavirus

    • 由来種

      Mouse

    • アプリケーション

      適用あり: ELISA, WBmore details

    • 種交差性

      交差種: SARS-CoV

    • 免疫原

      Recombinant full length protein corresponding to Human coronavirus SARS E2. Purified recombinant fragment of SARS-E2 glycoprotein precursor expressed in E. Coli.

    • ポジティブ･コントロール

      • SARS E2 Recombinant protein.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • バッファー

      Preservative: 0.05% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 精製度

      Protein G purified

    • 特記事項（精製）

      Purified from tissue culture supernatant.

    • ポリ/モノ

      モノクローナル

    • クローン名

      4A6C9

    • アイソタイプ

      IgG1

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab31951の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ELISA
    Use at an assay dependent concentration.
    WB
    1/2000 - 1/5000. Detects a band of approximately 36 kDa.
    特記事項
    ELISA
    Use at an assay dependent concentration.
    WB
    1/2000 - 1/5000. Detects a band of approximately 36 kDa.

    ターゲット情報

    • 関連性

      SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The complete sequence of the SARS virus release the coronavirus contains 25 open reading frames. SARS E2 glycoprotein precursor is a 139kDa glycoprotein. It contains a superantigen between residues 690 through 1050 which has relationship to T-cell Receptor alpha-beta V chain protein.

    画像

    • Western blot - Anti-SARS E2 antibody [4A6C9] - Coronavirus (ab31951)
      Western blot - Anti-SARS E2 antibody [4A6C9] - Coronavirus (ab31951)
      Lane 1 : MW Marker
      Lane 2 : Anti-SARS E2 antibody [4A6C9] - Coronavirus (ab31951) at 1/2000 dilution

      Lane 2 : SARS E2 Recombinant protein

      Observed band size: 36 kDa why is the actual band size different from the predicted?

    参考文献 (0)

    ab31951 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab31951 は論文での使用が確認できていません。

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    ab39839  SARS E protein peptide    Could it be used with Anti-SARS E2 antibody [4A6C9] (ab31951) and Anti-SARS Nucleoprotein antibody [BDI186] (ab28169) to do sandwich assay? I want one antigen (SARS) and two monoclonal antibodies for the test of sars sandwich assay. Could you give me some suggestions ?Thank you!

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    Answer

    Thank you for your enquiry. I am sorry to confirm we do not have any SARS antibodies that are tested in sandwich ELISA (sELISA). SARS E2 antibody [4A6C9] (ab31951) and SARS Nucleoprotein antibody [BDI186] (ab28169) are tested in indirect ELISA only. I can suggest to consider trying one of them in an indirect ELISA method. Sandwich ELISA procedures can be difficult to optimise and tested match pair antibodies should be used. Any tested matched pairs would be noted on the datasheet. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, I am sorry we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA. I am sorry we have no suitably tested antibodies for sELISA on this occasion. I can suggest having a look on the Biocompare website. They have an excellent antibody search facility which includes a lot of suppliers. Regrettably, I am also not able to confirm that the ab28169 is raised against the SARS nucleoprotein, so will not detect the ab39839 E2 protein peptide. However, the ab39839 peptide has been specifically used as the immunogen for ab31951 SARS E2 antibody [4A6C9], so these can be used together in indirect ELISA. I hope this will be helpful. Should you have any further questions, please do not hesitate to contact us. Good luck with your research.

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