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AB323003

Anti-SARS-CoV-2 S 抗体 [EPR24852-116] - Chicken IgY (Chimeric) - BSA and Azide free

Anti-SARS-CoV-2 S antibody [EPR24852-116] - Chicken IgY (Chimeric) - BSA and Azide free

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Chicken Recombinant Monoclonal SPIKE antibody. Carrier free. Suitable for WB, I-ELISA and reacts with SARS-CoV-2 samples.
2 Images
Indirect ELISA - Anti-SARS-CoV-2 S antibody [EPR24852-116] - Chicken IgY (Chimeric) - BSA and Azide free (AB323003)
  • I-ELISA

Lab

Indirect ELISA - Anti-SARS-CoV-2 S antibody [EPR24852-116] - Chicken IgY (Chimeric) - BSA and Azide free (AB323003)

This data was developed using ab323001, the same antibody clone in a different buffer formulation.

Indirect ELISA showing primary antibody ab323001 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) and Recombinant Human coronavirus SARS spike glycoprotein (Tagged, ab270844) at 1000 ng/ml. Binding of ab323001 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Chicken IgY H&L (HRP, ab6877) was used at 1 : 5000.

Western blot - Anti-SARS-CoV-2 S antibody [EPR24852-116] - Chicken IgY (Chimeric) - BSA and Azide free (AB323003)
  • WB

Lab

Western blot - Anti-SARS-CoV-2 S antibody [EPR24852-116] - Chicken IgY (Chimeric) - BSA and Azide free (AB323003)

This data was developed using ab323001, the same antibody clone in a different buffer formulation.

Lane 1 : Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active, His tagged) (ab273068) at 0.2 ug

Lane 2 : Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 ug

False colour image of Western blot : Rabbit monoclonal [EPR24852-116] to SARS-CoV-2 Spike Glycoprotein S1 Chicken IgY (Chimeric) (ab323001) staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (ab18184) loading control staining at 1/1000 dilution, shown in red. In Western blot, ab323001 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Chicken IgY H&L (HRP) preadsorbed (ab7118) at 1 : 10000 and Goat anti-Mouse IgG H&L 680RD at 1 : 20000.

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Chicken IgY (Chimeric) (<a href='/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s1-antibody-epr24852-116-chicken-igy-chimeric-ab323001'>ab323001</a>) at 1/500 dilution

Lane 1:

Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (<a href='/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-spike-glycoprotein-s1-active-ab273068'>ab273068</a>) at 0.2 µg

Lane 2:

Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-preadsorbed-ab7118'>ab7118</a>) at 1/10000 dilution

Lanes 1 - 2:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 135 kDa

false

Exposure time: 4min

関連する標識済み抗体及び組成の異なる製品 (1)

  • Unconjugated

    Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Chicken IgY (Chimeric)

Key facts

宿主種

Chicken

クローン性

Monoclonal

クローン番号

EPR24852-116

アイソタイプ

IgY

キャリアフリー

Yes

交差種

SARS-CoV-2

アプリケーション

WB, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }
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製品の詳細

This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab283942). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

ab323003 is the carrier free version of ab323001.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Thiophilic Resin
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information SARS-CoV-2 Spike Glycoprotein S1
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組合せ製品

Assay Kits

AB275299

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Abcam product promise

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