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リコンビナントRabMAb

Anti-SARS-CoV-2 nsp13 抗体 [EPR24838-18] - BSA and Azide free (ab286917)

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Flow Cytometry - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)
  • Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)
  • Western blot - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)
  • Indirect ELISA - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)
  • Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24838-18] to SARS-CoV-2 nsp13 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, Indirect ELISA, ICC/IF

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free
    SARS-CoV-2 nsp13 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24838-18] to SARS-CoV-2 nsp13 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, Indirect ELISA, ICC/IFmore details

  • 種交差性

    交差種: SARS-CoV-2

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Recombinant SARS-CoV-2 nsp13 protein (His-tagged). ICC/IF: HEK-293T cells transfected with a SARS-CoV-2 nsp13 expression vector containing a myc tag Flow cyto: HEK-293T cells transfected with a SARS-CoV-2 nsp13 expression vector containing a myc tag

  • 特記事項

    ab286922 is a carrier-free version of ab286909.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.4
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR24838-18

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab286917の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa.
Indirect ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa.
Indirect ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

画像

  • Flow Cytometry - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)
    Flow Cytometry - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)

    This data was developed using ab286909, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis 4% Paraformaldehyde fixed 90% methanol permeabilised HEK-293T (Human embryonic kidney epithelial cell) cells transfected with a SARS-CoV-2 nsp1 (Left) or SARS-CoV2 nsp13 (Right) expression vector containing a myc tag labelling SARS-CoV-2 nsp13 with ab286909 at 1/50 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) (ab150081) at 1/5000 dilution was used as the secondary antibody. Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895) was used to label transfected cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)
    Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)

    This data was developed using ab286909, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% TritonX-100 permeabilised HEK-293T (Human embryonic kidney 293) cells labelling Anti-SARS-CoV-2 nsp13 with ab286909 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) antibody at 1/1000 dilution.

    Confocal image showing cytoplasmic and nuclear staining in HEK-293T cells transfected with a SARS-CoV-2 nsp13 expression vector containing a myc tag, and no staining in HEK-293T cells transfected with a SARS-CoV-2 nsp1 expression vector containing a myc tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Myc-Tag Mouse mab (Alexa Fluor® 647) was used to counterstain Myc-Tag at 1/100 dilution (Red). The Nuclear counterstain was DAPI.

    Secondary antibody only control: AlexaFluor®488 Goat anti-Rabbit (ab150081) at 1/1000 dilution.

  • Western blot - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)
    Western blot - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)
    Lanes 1-4 : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution
    Lanes 5-8 : Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] (ab286909) at 1/1000 dilution

    Lanes 1 & 5 : Recombinant SARS-CoV-2 nsp13 protein (His-tagged) at 0.5 µg
    Lanes 2 & 6 : Recombinant SARS-CoV-2 nsp13 protein (His-tagged) at 0.2 µg
    Lanes 3 & 7 : Recombinant SARS-CoV2 nsp1 protein (His tagged) at 0.5 µg
    Lanes 4 & 8 : Recombinant SARS-CoV2 nsp1 protein (His tagged) at 0.2 µg

    Secondary
    Lanes 1-4 : Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/20000 dilution
    Lanes 5-8 : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution

    Observed band size: 70 kDa why is the actual band size different from the predicted?



    This data was developed using ab286909, the same antibody clone in a different buffer formulation.

    ab286909 was shown to bind specifically to SARS-CoV-2 nsp13 protein in western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    Blocking buffer: 5% milk in TBS-0.1% Tween® 20 (TBS-T)

  • Indirect ELISA - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)
    Indirect ELISA - Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)

    This data was developed using ab286909, the same antibody clone in a different buffer formulation.

    Indirect ELISA- Anti-SARS-CoV2 NSP13 antibody (ab286909)

    Indirect ELISA showing primary antibody ab286909 binding to recombinant SARS-CoV-2 nsp13 protein (His-tagged). Plates were coated with recombinant SARS-CoV-2 nsp13 protein (His-tagged) and recombinant SARS-CoV2 nsp1 protein (His-tagged) at 1000 ng/ml. Binding of ab286909 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution).

    Binding was detected using pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) at 1/2000 dilution.

  • Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)
    Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] - BSA and Azide free (ab286917)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab286917 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab286917 は論文での使用が確認できていません。

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