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リコンビナントRabMAb

Anti-SARS-CoV-2 nsp10 抗体 (ab286914)

Reviews (1) Submit a question
Western blot - Anti-SARS-CoV-2 nsp10 antibody [EPR24836-88] (ab286914)
  • Indirect ELISA - Anti-SARS-CoV-2 nsp10 antibody [EPR24836-88] (ab286914)
  • Anti-SARS-CoV-2 nsp10 antibody [EPR24836-88] (ab286914)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24836-8-8] to SARS-CoV-2 nsp10
  • Suitable for: WB, Indirect ELISA

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SARS-CoV-2 nsp10 antibody
    SARS-CoV-2 nsp10 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24836-8-8] to SARS-CoV-2 nsp10

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Indirect ELISAmore details

  • 種交差性

    交差種: SARS-CoV-2

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Recombinant SARS-CoV-2 nsp10 protein.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59.94% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR24836-8-8

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab286914の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000. Detects a band of approximately 19 kDa.
Indirect ELISA
Use at an assay dependent concentration.

0.016 - 1000 ng/mL
特記事項
WB
1/1000. Detects a band of approximately 19 kDa.
Indirect ELISA
Use at an assay dependent concentration.

0.016 - 1000 ng/mL

画像

  • Western blot - Anti-SARS-CoV-2 nsp10 antibody [EPR24836-88] (ab286914)
    Western blot - Anti-SARS-CoV-2 nsp10 antibody [EPR24836-88] (ab286914)
    Lanes 1-2 : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution
    Lanes 3-4 : Anti-SARS-CoV-2 nsp10 antibody (ab286914) at 1/1000 dilution

    Lanes 1 & 3 : Recombinant SARS-COV-2 nsp10/nsp16 methyltransferase protein (His-tagged) at 0.5 µg
    Lanes 2 & 4 : Recombinant SARS-COV-2 nsp10/nsp16 methyltransferase protein (His-tagged) at 0.2 µg

    Secondary
    Lanes 1-2 : Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/20000 dilution
    Lanes 3-4 : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution

    Observed band size: 19 kDa why is the actual band size different from the predicted?



     Lanes 1 - 2: Red – loading control ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) observed at 19 kDa. The band observed at 36kDa represents NSP16.

    Lanes 3 - 4: Green – ab286914 observed at 19 kDa (NSP10).

    ab286914 was shown to bind specifically to SARS-CoV-2 NSP10 protein in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    Blocking buffer: 3% milk in TBS-0.1% Tween® 20 (TBS-T).

  • Indirect ELISA - Anti-SARS-CoV-2 nsp10 antibody [EPR24836-88] (ab286914)
    Indirect ELISA - Anti-SARS-CoV-2 nsp10 antibody [EPR24836-88] (ab286914)

    Indirect ELISA- Anti-SARS-CoV2 NSP10 antibody (ab286914)

    Indirect ELISA showing primary antibody ab286914 binding to recombinant SARS-COV-2 nsp10/nsp16 methyltransferase protein (His-tagged). Plates were coated with recombinant SARS-COV-2 nsp10/nsp16 methyltransferase protein (His-tagged) and recombinant SARS-COV-2 nsp9 protein (His-tagged) at 1000 ng/ml. Binding of ab286914 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution).

    Binding was detected using pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) at 1/2000 dilution.

  • Anti-SARS-CoV-2 nsp10 antibody [EPR24836-88] (ab286914)
    Anti-SARS-CoV-2 nsp10 antibody [EPR24836-88] (ab286914)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab286914 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab286914 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

Western blot abreview for Anti-SARS-CoV-2 nsp10 antibody [EPR24836-8-8]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Caco-2 human colorectal adenocarcinoma cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris NuPAGE)
Loading amount
40 µg
Specification
Caco-2 human colorectal adenocarcinoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Nicola Goodman

Verified customer

投稿 Apr 14 2022

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